| 高俊薇,李雅娟,庄辉,李杰,王佳,董庆鸣,陈雅洁,牛俊奇,马为民,赵伟,赵保安,钟金群.中国11城市乙型肝炎病毒慢性感染者中乙型肝炎病毒基因型分布[J].Chinese journal of Epidemiology,2007,28(4):315-318 |
| 中国11城市乙型肝炎病毒慢性感染者中乙型肝炎病毒基因型分布 |
| Distribution of hepatitis B virus genotypes in patients with chronic hepatitis B virus infection among 11 cities of China |
| Received:February 07, 2007 |
| DOI： |
| KeyWord: 乙型肝炎病毒 聚合酶链反应 序列分析 基因型 |
| English Key Word: Hepatitis B virus Polymerase chain reaction Sequencing Genotype |
| FundProject:国家“十五”科技攻关课题资助项目(2004BA718802)；北京市科委课题资助项目(H020920020190) |
| Author Name | Affiliation | E-mail | | GAO Junwei | Department of Microbiology, peking University Health Sience Center, Beijing 100083, China | | | LI Yajuan | Department of Microbiology, peking University Health Sience Center, Beijing 100083, China | | | ZHUANG Hui | Department of Microbiology, peking University Health Sience Center, Beijing 100083, China | zhuangbmu@126．com | | LI Jie | Department of Microbiology, peking University Health Sience Center, Beijing 100083, China | jieli69@263．Net | | WANG Jia | Department of Microbiology, peking University Health Sience Center, Beijing 100083, China | | | DONG Qingming | 北京地坛医院 | | | CHEN Yajie | 长春市北方肝胆医院 | | | NIU Junqi | 吉林大学第一附属医院 | | | MA Weimin | 北京大学深圳医院 | | | ZHAO Wei | 南京市第二医院 | | | ZHAO Baoan | 湖北省汉川市疾病预防控制中心 | | | ZHONG Jinqun | 广东省清远市疾病预防控制中心 | |
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| Abstract: |
| 目的<\b> 了解中国乙型肝炎病毒(HBV)慢性感染者中HBV基因型的分布情况.方法<\b> 收集北京、清远、深圳、石家庄、汉川、南京、长春、济南、聊城、宁波和温州市共1214份HBV DNA阳性的慢性HBV感染者血清样本,用型特异性引物聚合酶链反应法(PCR)进行基因型测定,并对其中部分样本以PCR产物直接测序验证.结果<\b> 在1214份血清中,检测到A基因型9例,占0.7%;B基因型345例,占28.4%;C基因型709例,占58.4%;B、C混合基因型(B+C)151例,占12.4%.未检测到其他基因型.北方地区(长春、北京、石家庄市等)慢性HBV感染者中,C基因型比例较高,分别为58.2%、67.5%和63.6%,山东省聊城和济南市的C基因型比例分别高达90.2%和87.9%.随地理位置南移,B基因型比例逐渐增加,广东省清远和深圳市的B基因型比例分别为71.4%和63.6%.结论<\b> HBV基因型分布有明显地区差异.在中国慢性HBV感染者中,HBV C和B基因型为主要流行株.北方地区以C基因型为优势株,而南方地区则B基因型较为多见. |
| English Abstract: |
| Objective<\b> To investigate the distributionof hepatitis B virus(HBV)genotypes in patients with chronic HBV infection among 11 cities of China.Methods<\b> A total of 1214 serum samples from patients with chronic HBV infection were collected in 1 1 cities of China，including Be巧ing，Qingyuan,Shenzhen，Sh巧iazhuang，Hanchuan，Nanjing，Changchun，Liaocheng，Jinan，Ningbo and Wenzhou.GenotypeS of the 1214 HBV strains were identified by PCR method with type specific primers.Parts of the results were confirmed by direct sequencing analysis of PCR products.Results<\b> Among the 1214 patients with chronic HBV infection，0.7％(9／1214)were genotype A，28.4％(345／1214)genotype B，58.4％(709／ 1214)genotype C，and 12.4％(151／1214)genotype B and genotype C mixed infection.Noother genotypes were found.Genotype C was predominant in the northern part of China，such as Changchun，Beijing,Shijiazhuang，while genotype B was more commonly seen in south of China.71.4％(20／28)for patients from Qingyuan and 63.6％(70／110)from shenzhen were infected with genotype B.conclusion HBV genotypes had distinct geographic distribution.Genotype B and C the predominant strains in patients with chronic HBV infection in China.Genotype C was predominantly identified in the northern part of China versus genotype B the south. |
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