广西壮族自治区隆安县乙型肝炎疫苗全程接种后的免疫记忆研究

李艳萍; 李荣成; 方孔雄; 李河民; 胡忠玉; 何鹏; 吴小音; 张瑞; 梁争论; 庄辉

Abstract

李艳萍,李荣成,方孔雄,李河民,胡忠玉,何鹏,吴小音,张瑞,梁争论,庄辉.广西壮族自治区隆安县乙型肝炎疫苗全程接种后的免疫记忆研究[J].Chinese journal of Epidemiology,2007,28(4):319-321

广西壮族自治区隆安县乙型肝炎疫苗全程接种后的免疫记忆研究

Studies on the status of immune memory after completion of hepatitis B vaccination

Received:February 01, 2007

DOI：

KeyWord: 乙型肝炎病毒免疫记忆加强免疫

English Key Word: Hepatitis B virus Immune memory BOOster immunization

FundProject:国家“十五”科技攻关课题资助项目(2004BA718802)

Author Name	Affiliation
LI Yanping	Guangxi zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530021, China
LI Rongcheng	Guangxi zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530021, China
FANG Kongxiong	隆安县疾病预防控制中心
LI Hemin	中国药品生物制品检定所
HU Zhongyu	中国药品生物制品检定所
HE Peng	中国药品生物制品检定所
WU Xiaoyin	中国药品生物制品检定所
ZHANG Rui	中国药品生物制品检定所
LIANG Zhenglun	中国药品生物制品检定所
ZHUANG Hui	北京大学医学部微生物学系

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Abstract:

目的<\b> 了解接种全程乙型肝炎(乙肝)疫苗后的免疫记忆情况.方法<\b> 1987-1989年出生时接种乙肝血源疫苗的1201名新生儿,以及1996-1999年出生时接种乙肝酵母重组疫苗的2484名新生儿,于2005年随访时检测乙肝表面抗原(HBsAg)、表面抗体(抗-HBs)和核心抗体(抗-HBc),结果<\b>959名3项乙肝病毒(HBV)标志物均阴性,其中228名接种乙肝血源疫苗,731名接种乙肝酵母重组疫苗,于加强免疫1针乙肝酵母重组疫苗后15 d时检测其抗-HBs.此外,随机选择11名加强免疫后无应答和22名有应答者,应用酶免疫斑点法(ELISPOT)测定白细胞介素-2(IL-2).有初次免疫后抗-HBs定量检测资料者190名,比较其初次免疫和加强免疫后抗-HBs水平.结果<\b> 加强免疫后,79.82%接种乙肝血源疫苗者抗-HBs阳转,几何平均滴度(GMT)为325.69 mIU/ml;95.62%接种乙肝酵母重组疫苗者抗-HBs阳转,GMT为745.18 mIU/ml.加强免疫后所产生的抗-HBs水平与初次免疫后抗体滴度有关.加强免疫后抗-HBs阳转者的IL-2阳性率(40.91%)也高于无应答者(P＜0.01).结论<\b> 在乙肝疫苗初次免疫后,抗-HBs转阴者中,大部分仍具有免疫记忆,仅少部分在长期随访中丧失免疫记忆.因此,在高流行地区,对丧失免疫记忆者应进行乙肝疫苗加强免疫。

English Abstract:

Objective<\b> To study the immune memory in vaccinees after the completion of a full schedule heDatitis B immunization.Methods<\b> One thousand and two hundred one infants born in 1987-1989 were immunized with 3 doses of plasma derived hepatitis B vaccine，while 2484 newbom babies during 1996-1999 were injected with 3 doses of the yeast recombinant hepatitis B vaccine.All of the infants under observation were tested for HBsAg，anti-HBs and anti-HBc，in 2005.Of 959 individuals negative for anti-HBs(<10 mIU／m1)，HBsAg and anti-HBc，228 were immunized with plasma-derived vaccine and 731 with veast recombinant vaccine after birth.A1l of them were detected for anti一HBs 15 days after a booster of 10 μg yeast recombinant vaccine.In addition，interleukin一2(IL一2)was detected in 11 non-responders and 22 responders after boostering，using an enzvme-linked immunospot(ELISPOT).The anti。HBs levels of 190 individuals(91 with Dlasma derived vaccine and 99 with yeast recombinant vaccine)who had had quantitative data on their antibody status after the primary hepatitis B vaccination，were compared with that after the boostering.Results<\b> Among the individuals who received plasma derived vaccine 16-18 years ago，79.82％of them showed the signs of immune memory after one booster，with a geometric mean titer (GMT)of 325.69 mIU／mI.Of the individuaIs who received the yeast recombinant vaccine 6-9 vears ago，95.62％showed immune memorv after one booster，with its GMT of 745.18 mIU／m1.Anti-HBs levels induced by the booster were associated with that after the primary immunization.The positive rate of IL一2 was 40.91％in subiects with good immune memory.However，IL一2 was not detected in rlon-responders after the booster(P<0.01).Conclusion<\b> IⅥost of the individuals who had received a completed schedule of primary hepatitis B vaccination and seroconverted from anti-HBs positive to negative，showed the signs of having immune mcmorv after the b00ster.0nly a small proportion of the vaccinees had lost their immune memory during the 10ng term fonow-up period，suggesting that these individuals should receive a booster of hepatitis B vaccine in the highlv endemic areas of hepatitis B.

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