成人接种乙型肝炎疫苗后细胞免疫应答的动态变化

胡忠玉; 朱凤才; 何鹏; 刘社兰; 张瑞; 方鑫; 翟祥军; 邱少辉; 梁争论; 汪华; 李河民; 庄辉

Abstract

胡忠玉,朱凤才,何鹏,刘社兰,张瑞,方鑫,翟祥军,邱少辉,梁争论,汪华,李河民,庄辉.成人接种乙型肝炎疫苗后细胞免疫应答的动态变化[J].Chinese journal of Epidemiology,2007,28(4):326-330

成人接种乙型肝炎疫苗后细胞免疫应答的动态变化

Study on the kinesis of cellular immunity in adults vaccinated with recombinant hepatitis B vaccine

Received:February 16, 2007

DOI：

KeyWord: 酶联免疫斑点法外周血单个核细胞斑点形成细胞计数

English Key Word: Enzyme—linked immunospot Peripheral b100d m。nonuclear cells spot forming cell

FundProject:国家“十五”科技攻关课题资助项目(2004BA718802)

Author Name	Affiliation	E-mail
HU Zhongyu	National Institute for the Control of Pharmaceutical and Bionlogical Products, Beijing 100050, China
ZHU Fengcai	江苏省疾病预防控制中心
HE Peng	National Institute for the Control of Pharmaceutical and Bionlogical Products, Beijing 100050, China
LIU Shelan	江苏省疾病预防控制中心
ZHANG Rui	National Institute for the Control of Pharmaceutical and Bionlogical Products, Beijing 100050, China
FANG Xing	National Institute for the Control of Pharmaceutical and Bionlogical Products, Beijing 100050, China
ZHAI Xiangjun	江苏省疾病预防控制中心
QIU Shaohui	National Institute for the Control of Pharmaceutical and Bionlogical Products, Beijing 100050, China
LIANG Zhenglun	National Institute for the Control of Pharmaceutical and Bionlogical Products, Beijing 100050, China	1zlun@yahoo com
WANG Hua	江苏省疾病预防控制中心
LI Hemin	National Institute for the Control of Pharmaceutical and Bionlogical Products, Beijing 100050, China
ZHUANG Hui	北京大学医学部微生物学系

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Abstract:

目的<\b> 探讨人体接种乙型肝炎(乙肝)疫苗后细胞免疫的动态变化,了解疫苗介导的细胞免疫应答与体液免疫应答的关联.方法<\b> 8例成人按0、1、2月程序接种乙肝疫苗,于首针免疫后3、8、21、34和65 d采静脉血,分离外周血单个核细胞(PBMC),对PBMC进行细胞分选,获得纯度95%以上的CD4^{+<\sup>和CD8^{+<\sup> T淋巴细胞,应用酶联免疫斑点法(ELISPOT)测定PBMC、CD4^{+<\sup>和CD8^{+<\sup> T淋巴细胞体外刺激后所产生的细胞因子IFN-γ、IL-2和IL-4的斑点数(SFC),并收集乙肝疫苗免疫前(0 d)和免疫后3、8、21、30、34、60、65和150 d血样,测定乙肝表面抗体(抗-HBs).同时设立阴、阳性对照各2例.结果<\b> 人接种乙肝疫苗后,CD4^{+<\sup>、CD8^{+<\sup> T淋巴细胞产生不同细胞因子趋势和强度不同:IFN-γ(由CD8^{+<\sup>、CD4^{+<\sup> T淋巴细胞产生)出现较早,且较为稳定,而CD4^{+<\sup> T淋巴细胞产生的IL-2和IL-4出现较晚,但其升高与第2、3针乙肝疫苗免疫有关;免疫后IL-4阳转率与抗-HBs阳转率呈显著正相关,IL-2、IL-4的SFC与抗-HBs滴度也呈显著正相关.结论<\b> 成人接种乙肝疫苗后可早期检测出以IFN-γ为主的细胞免疫,IL-4和IL-2阳转与抗-HBs升高有关.}}}}}}}}}

English Abstract:

Objective<\b> To evaluate the kinesis of cellular immunity in adults who were vaccinated with yeast recombinant heDatitis B(rHB)vaccine and the correlation between cellular and humoral immune responses induced by the vaccine.IⅥethods Eight adults were vaccinated with rHB vaccine according to 0，1，2 month schedule.The pe“pheral blood mononuclear cens(PBMCs)were collected at the 3，8，21，34 and 65 days after the first dose.The high purity of CD4^{+<\sup> and CD8^{+<\sup> T ceUs obtained by sorting from PBMCs were restimulated with recombinant hepatitis B surface antigens(rHBsAg)or peptides.The spot forming cell(SFC)of IFN-丫，IL-2 and IL-4 of CD4^{+<\sup> and CD8^{+<\sup>T cells were detected by enzyme-linked immunospot(EI.ISPOT).Results<\b> The characte“stics of IFN-丫，IL-2 and IL-4 of CD4^{+<\sup> and CD8^{+<\sup> T cells appeared different after immunization with rHB vaccine.IFN-7 of CD8^{+<\sup> and CD4^{+<\sup> T cells could be detected early with stable SFC，while the IL-2 and IL-4 of CD4^{+<\sup>T cells appeared late but increased after the second and third dose of vaccination.The positive rate of IL-4 of CD4^{+<\sup>T cells were significantly correlated with the Dositive rate of anti-HBs.w hile the SFCs of IL-4 and IL.2 of CD4^{+<\sup>T cells were also significantlv related to the titers of anti-HBs.Conclusion<\b> IFN-7 could be detected early after rHB vaccination in adults.and the Dositive rates of IL-4 and II.-2 were correlated with that of anti-HBs.}}}}}}}}}}}

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