| 裘莉佩,潘登,徐炜烽,周华,魏泽庆,俞云松.鲍曼不动杆菌碳青霉烯酶基因型及分子流行病学研究[J].Chinese journal of Epidemiology,2007,28(4):381-384 |
| 鲍曼不动杆菌碳青霉烯酶基因型及分子流行病学研究 |
| Study on the carbapenemase genotype and molecular epidemiology of Acinetobacter baumannii |
| Received:August 30, 2006 |
| DOI： |
| KeyWord: 鲍曼不动杆菌 脉冲场凝胶电泳 流行病学，分子 |
| English Key Word: Acinetobacter baumannii Pulsed—field gel electrophoresis Epidemio1ogy,molecular |
| FundProject:国家自然科学基金资助项目(30370073) |
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| Abstract: |
| 目的<\b> 研究宁波地区3家综合性医院临床分离亚胺培南耐药鲍曼不动杆菌的耐药性、同源性及碳青霉烯酶基因型.方法<\b> 收集宁波市李惠利医院、宁波市第一人民医院、宁波市第二人民医院碳青霉烯类抗生素耐药鲍曼不动杆菌28株.琼脂稀释法和E-test法测定14种抗菌药物的最小抑菌浓度值;脉冲场凝胶电泳(PFGE)分析菌株同源性;PCR及克隆测序分析碳青霉烯酶基因型.结果<\b> 28株鲍曼不动杆菌对多粘菌素E耐药率最低为4.7%,头孢哌酮/舒巴坦为87%,其余抗菌药物耐药率均高于90%耐药.PFGE分型共分为4型,以A、B两型为主.28株鲍曼不动杆菌中均产OXA-23碳青霉烯酶基因,未检测到VIM、IMP型金属酶基因.结论<\b> 宁波地区碳青霉烯抗生素耐药鲍曼不动杆菌均产OXA-23型碳青霉烯酶,3家医院均有克隆播散流行. |
| English Abstract: |
| Objective<\b> To investigate antibiotic resistance，clonal relatedneSs and carbapenemase genotype among carbapenem-resistant Acinetobacter baumannii collected from 3 comprehensive hospitals in Ningbo city，Zhejiang province.Methods<\b> 28 strains of carbapenem resistant Acinetobacter baumannii were collected from Ningbo Li Hui-li Hospital，Ningbo First Hospital，and Ningbo Second Hospital.The minimum inhibitory concentrations(MIC)of these strains were examined by agrar dilution and E-test method.Homology of these isolates was analyzed by pulse-field gel electrophoresis(PFGE)and Genotype of carbapenemaSeS were analyzed by PCR and verified by DNA sequencing.ResultS 28 strains of Acinetobacter baumannii were highly resistant to an of the antibiotics except p01ymyxin E.They were cIassified into 4 clones based on PFGE pattern.Clone A and B had been spreading widely.All of the 28 strains produced carbapenemases which were confirmed as 0XA-23 by PCR and sequencing，Metallo-beta- lactamase was not detected in any of the iSoluates.Conclusion<\b> All of the carbapenem.resistant Acinetobacter baumannii collected from Ningbo were producing OXA-23 carbapenemase，suggesting that the transmission of clones had occurred in the 3 hospitals. |
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