| 祝仁发,宫照龙,叶长芸,崔志刚,金东,冉陆,许珂,王连秀,陈伟伟.中国八省市食品来源单核细胞增生李斯特菌的脉冲场凝胶电泳分析[J].Chinese journal of Epidemiology,2007,28(5):464-467 |
| 中国八省市食品来源单核细胞增生李斯特菌的脉冲场凝胶电泳分析 |
| Study on molecular typing by pulsed-field gel electrophoresis of food Listeria monocytogenes isolates from eight province(municipalities)of China |
| Received:January 10, 2007 |
| DOI： |
| KeyWord: 单核细胞增生李斯特菌 脉冲场凝胶电泳分型 |
| English Key Word: Listeria monocytogenes Pulsed-field gel electrophoresis |
| FundProject:国家自然科学基金资助项目(30470095) |
| Author Name | Affiliation | E-mail | | ZHU Ren-fa | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | GONG Zhao-long | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | YE Chang-yun | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | yechangyun@icdc.cn | | CUI Zhi-gang | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | JIN Dong | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | RAN Lu | 中国疾病预防控制中心营养与食品安全所 | | | XU Ke | 杭州市疾病预防控制中心 | | | WANG Lian-xiu | 北京市昌平区疾病预防控制中心 | | | CHEN Wei-wei | 福建省疾病预防控制中心 | |
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| Abstract: |
| 目的 采用国际标准化的脉冲场凝胶电泳(PFGE)分析方法对单核细胞增生李斯特菌(Lm)进行基因分型，初步建立中国Lm的基因分型数据库。方法 按照标准化的Lm-PFGE方法对来自国内8个省市、多种食品来源的118株Lm进行了PFGE分型。结果 118株Lm分成了39种带型，其中GX6A160004型有37株菌，占分析菌株的31。36%，是主要型别。结论 初步建立了中国单核细胞增生李斯特菌用于网络监测分析(PulseNet China)的基础数据库，发现同型菌株在全国多个地区广泛存在。 |
| English Abstract: |
| Objective To analyse the molecular types of Listeria(L.)monocytogenes，and to construct the standard China L. monocytogenes pulsed-field gel electrophoresis(PFGE) subtyping database,using the international standardized L.monocytogenes-PFGE protocol. Methods 118 L.monocytogenes strains isolated from 8 provinces or municipalities of China were subtyped according to L.monocytogenes-PFGE protocol. Results 118 strains of L. monocytogenes were divided into 39 subtypes. In the 39 subtypes, 37 strains(31.36)were GX6A160004 pattern, showing it was the predominant Lm subtype in China. Conclusion Data from molecular typing suggested that the predominant Lm strains were distributed in different regions of China. PulseNet China-Lm database was constructed which was valuable for the molecular subtyping-based surveillance of L.monocytogenes. |
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