| 张文义,江佳富,姚昆,吴晓明,左曙青,詹琳,褚宸一,赵秋敏,张泮河,杨红,曹务春.中国大林姬鼠携带Amur类汉坦病毒及其分子生物学特征分析[J].Chinese journal of Epidemiology,2007,28(5):482-486 |
| 中国大林姬鼠携带Amur类汉坦病毒及其分子生物学特征分析 |
| Identification of Amur like virus in Apodemus peninsulae and its molecular characteristics in China |
| Received:November 08, 2006 |
| DOI： |
| KeyWord: 汉坦病毒 Amur病毒 大林姬鼠 |
| English Key Word: Hantavirus Amur like virus Apodemus peninsulae |
| FundProject:国家自然科学基金资助重大项目(30590374);军队"十一五"计划立项课题(06MA337);北京市自然科学基金资助重点项目(06G0668) |
| Author Name | Affiliation | E-mail | | ZHANG Wen-yi | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | JIANG Jia-fu | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | YAO Kun | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | WU Xiao-ming | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | ZUO Shu-qing | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | ZHAN Lin | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | CHU Chen-yi | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | ZHAO Qiu-min | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | ZHANG Pan-he | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | YANG Hong | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | | | CAO Wu-chun | State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China | caowc@nic.bmi.ac.cn |
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| Abstract: |
| 目的 确定中国大林姬鼠是否存在Amur类汉坦病毒及其分子生物学特征。方法 应用RT-PCR法扩增大林姬鼠肺组织中汉坦病毒M基因片段和S基因全长，目的 片段经测定其核苷酸序列后，用DNASTAR软件进行同源性比对和系统发育分析。结果 从东北大林姬鼠肺标本JilinAP06中分别扩增出长度383 bp(M基因)和1696 bp(S基因)大小的目的cDNA片段，其S基因全长由1696个核苷酸组成，完整的开放阅读框从37位核苷酸至1323位核苷酸，共1287个核苷酸，编码429个氨基酸的蛋白。JilinAP06 S基因与B78、Liu株同源性最高，与H5株同源性次之，与AP63、AP61和AP1371同源性为91。0%～91。7%，与76-118同源性为81。0%。S全序列基因推导的氨基酸序列与其他汉坦病毒已知序列比较显示:JilinAP06与AP1371同源性最高(98。4%)，与AP61同源性为98。1%，与AP63和Liu、B78和H5株同源性为97。2%～97。9%，与76-118同源性为95。1%。系统发育分析结果显示:JilinAP06与Amur类汉坦病毒构成一个相对独立的分支。M基因片段同源性比较和系统发育分析结果与基于S基因全长得到的结果一致。结论 中国大林姬鼠存在Amur类病毒，大林姬鼠很可能为Amur类病毒的自然宿主和人群Amur类病毒感染的重要来源。 |
| English Abstract: |
| Objective To confirm the existence of Amur-like viruses in Apodemus peninsulae in China, and to understand the molecular characteristics of these viruses. Methods Total RNA was extracted from lungs of A.peninsulae captured in Jilin of Northeast China with Trizol. reagent. Complete S and partial M segments of Amur virus were amplified and sequenced. Phylogenetic analyses on multiple nucleotide sequences were performed with the Clustal method and DNASTAR software. Results 383 by cDNA of M segment and 1696 by of S segment of Amur like virus were recovered from lung tissue of A. peninsulae，named JilinAP06. The full-length of its S gene comprised of 1696 nucleotides with ORF including 1287 nucleotides and encoding a protein which comprised 429 amino acids. The phylogenetic analysis of this sample with other hantaviruses revealed that the complete S and partial M segment sequence of JilinAP06 both were closely related to those Amur viruses such as AP63，AP61，AP1371 and AP1168 found in A.peninsulae from Far East region of Russia and B78 strain, Liu strain and HS strain, which were all from Chinese patients. The complete S and partial M segment sequence of JilinAP06 had only 81.0 % identities with the nucleotide sequences of HV prototype 76-118 strain. Conclusion Amur-like viruses did exist in A. peninsulae from Northeasern China while A. peninsulae might be the natural reservoir of Amur-like viruses in China and was the important infectious source to HFRS patients which were caused by Amur-like viruses. |
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