| 兰全学,扈庆华,石晓路,王冰,林一曼,张顺祥,程锦泉,阚飙,徐建国.深圳市1993-2002年霍乱弧菌的脉冲场凝胶电泳分析[J].Chinese journal of Epidemiology,2007,28(5):491-494 |
| 深圳市1993-2002年霍乱弧菌的脉冲场凝胶电泳分析 |
| Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993 to 2002 |
| Received:August 31, 2006 |
| DOI： |
| KeyWord: 霍乱弧菌 脉冲场凝胶电泳 分子分型 |
| English Key Word: Vibrio cholerae Pulsed-field gel electrophoresis Molecular subtyping |
| FundProject:国家自然科学基金资助项目(30300281),国家科技攻关计划(2003BA712A05-04),广东省卫生厅资助项目(A2003709) |
| Author Name | Affiliation | E-mail | | LAN Quan-xue | Shenzhen Center for Disease Control and Prevention, Shenxhen 518020, China | | | HU Qing-hua | Shenzhen Center for Disease Control and Prevention, Shenxhen 518020, China | huqinghua03@163.com | | SHI Xiao-lu | Shenzhen Center for Disease Control and Prevention, Shenxhen 518020, China | | | WANG Ring | Shenzhen Center for Disease Control and Prevention, Shenxhen 518020, China | | | LIN Yi-man | Shenzhen Center for Disease Control and Prevention, Shenxhen 518020, China | | | ZHANG Shun-xiang | Shenzhen Center for Disease Control and Prevention, Shenxhen 518020, China | | | CHENG Jin-quan | Shenzhen Center for Disease Control and Prevention, Shenxhen 518020, China | | | KAN Riao | 中国疾病预防控制中心传染病预防控制所 | | | XU Jian-guo | 中国疾病预防控制中心传染病预防控制所 | |
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| Abstract: |
| 目的 分析深圳市1993-2002年霍乱弧菌菌株的相关性。方法 60株霍乱弧菌基因组DNA经Not Ⅰ酶切，通过脉冲场凝胶电泳获得电泳图谱，利用BioNumerics软件对图谱进行聚类分析。结果 60株霍乱弧菌被分为39种脉冲场凝胶电泳图谱，聚类分析将大部分菌株分为A、B两群。结论 深圳地区霍乱弧菌存在紧密相关的流行克隆群。霍乱弧菌的脉冲场凝胶电泳分析，有助于霍乱的主动监测和传染来源追踪。 |
| English Abstract: |
| Objective To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002. Methods. Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not 1 and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis(PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software. Results 39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B. Conclusion The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae. |
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