| 朱汝南,钱渊,赵林清,邓洁,王芳,孙宇,廖斌.北京地区2004-2006年婴幼儿急性呼吸道感染中人偏肺病毒感染的研究[J].Chinese journal of Epidemiology,2007,28(7):679-682 |
| 北京地区2004-2006年婴幼儿急性呼吸道感染中人偏肺病毒感染的研究 |
| Surveillance on human metapneumovirus in infants and children with acute respiratory infections in Beijing, from 2004 to 2006 |
| Received:March 26, 2007 Revised:June 26, 2012 |
| DOI： |
| KeyWord: 人偏肺病毒 婴幼儿 急性呼吸道感染 |
| English Key Word: Human metapneumovirus Infants and young children Acute respiratory infections |
| FundProject:国家自然科学基金资助项目(30570080);北京市自然科学基金资助项目(052020);北京市科委新星计划资助项目(2004B34) |
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| Abstract: |
| 目的 了解北京地区急性呼吸道感染婴幼儿中人偏肺病毒(hMPV)的感染情况.方法 采集2004年7月至2006年6月首都儿科研究所附属儿童医院门诊和住院呼吸道感染患儿的咽拭子和鼻咽洗液临床标本3330份,进行细胞培养和间接免疫荧光检测病毒;同时提取标本中病毒RNA后经逆转录聚合酶链反应扩增位于hMPV M基因的片段,经琼脂糖凝胶电泳检测扩增片段.结果 从3330份临床标本中检测到110份hMPV阳性标本,阳性率3.3%.hMPV感染的患儿男女比例为1.5∶1,其中<1岁年龄组患儿占46.4%(51/110),1～岁年龄组患儿占11.8%(13/110),2～5岁年龄组患儿占33.6%(37/110),>5岁年龄组患儿占8.2%(9/110).hMPV感染患儿的临床症状以肺炎占的比例最大,为44.5%(49/110);其次是诊断为上呼吸道感染(22.7%,25/110);诊断为毛细支气管炎的占10.9%(12/110),其中诊断支气管炎占7.3%(8/110).2004年7月至2005年6月期间几乎每个月份(除2005年5月和6月)都检出hMPV感染的阳性标本,未出现明显的流行高峰.而2005年7月至2006年6月期间hMPV出现了较为明显的流行,于2006年4月为最高峰,阳性检出率为17.1%.110例hMPV感染的临床标本中有6份标本存在与其他呼吸道病毒的合并感染.hMPV和同期的人呼吸道合胞病毒的感染高峰没有重叠.结论 hMPV是北京地区婴幼儿急性呼吸道感染(尤其是下呼吸道感染)的重要病毒病原之一,其感染对2岁以下婴幼儿威胁更大. |
| English Abstract: |
| Objective To understand the prevalence of human metapneumovirus(hMPV) infection in infants and young children in Beijing,China.Methods Gene fragment from hMPV was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with the primer pair located on the membrane (M) encoding gene.RNAs were extracted by Trizol from 3330 specimens collected from outpatients and inpatients with acute respiratory infections and visited the affiliated Children's Hospital from July 2004 to June 2006.These samples had been tested for conventional respiratory viruses including RSV,influenza A and B,parainfluenzaⅠ,Ⅱ,Ⅲand adenovirus by indirect immunofluoresence assay as well as virus isolation before RT-PCR for hMPV.Results Out of the 3330 clinical samples,110(3.3%) were found positive to hMPV.The ratio of male to female among those positive to hMPV was 1.5:1.Fifty-one out of 110 positive (46.4 96,51/110) specimens on hMPV were from children under one year olds,13 (11.8 %,13/ 110) were from 1 to 2 year olds,37 (33.6%,37/110)were from 2 to 5 years of age,and 9 (8.2%,9/110) among children over 5 years of age.hMPV was associated with:pneumonia in 44.5% (49/110) of the cases;upper respiratory infections in 22.7% (25/110);hronchiolitis in 10.9% (12/110);and bronchitis in 7.3% (8/110).hMPV was detected almost in every month from July 2004 to June 2005 in sporadic cases whereas a peak was noticed in April 2006 during the period from July 2005 to June 2006.Among 110 positive samples,6 (5.5 %) were also positive for other viruses including 3 as Influenza B,2 as RSV and 1 as Parainfluenza 3,suggesting that these children were co-infected with hMPV and other respiratory viruses. The peak of hMPV prevalence did not overlap with that of RSV during this period.Conclusion Our findings suggested that hMPV had been one of the important agents causing acute respiratory infections especially severe lower respiratory infections like pneumonia and bronchiolitis in pediatric patients in Beijing. Infants and young children under two years of age seemed to be more susceptible to hMPV. |
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