| 杨小冉,刘起勇,崔步云,王连秀,彭智会,任东升.间接酶联免疫吸附试验检测北京市昌平地区体检人群中汉赛巴尔通体抗体[J].Chinese journal of Epidemiology,2007,28(7):688-691 |
| 间接酶联免疫吸附试验检测北京市昌平地区体检人群中汉赛巴尔通体抗体 |
| Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in Changping, Beijing |
| Received:October 25, 2006 Revised:June 05, 2012 |
| DOI： |
| KeyWord: 汉赛巴尔通体 间接酶联免疫吸附试验 |
| English Key Word: Bartonella henselae Direct enzyme linked immunosorbent assay |
| FundProject:国家自然科学基金资助项目(30371246) |
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| Abstract: |
| 目的 调查北京市昌平地区健康体检人群血清汉赛巴尔通体抗体阳性情况.方法 用间接酶联免疫吸附试验(ELISA)和免疫荧光抗体(IFA)试剂盒检测体检人群血清汉赛巴尔通体抗体情况.结果 以IFA为参考标准,间接ELISA方法的灵敏度为70.6%,特异度为91.6%;阳性预测值为82.2%(60/73),阴性预测值为84.9%.ELISA共检测了357份健康体检人群血清,汉赛巴尔通体抗体阳性率为34.5%,IFA共检测了239份体检血清,其汉赛巴尔通体抗体阳性率为35.6%.结论 间接ELISA方法对于检测汉赛巴尔通体感染是一种快速、敏感、特异的方法,昌平地区健康体检人群中存在汉赛巴尔通体抗体阳性的情况. |
| English Abstract: |
| Objective To detect Bartonella henselae IgG antibody among healthy people in Changping,Beijing.Methods Using indirect enzyme linked immunosorbent assay (ELISA) and immunofluorescence antibody assay(IFA) to detect IgG antibody of Bartonella henselae among human beings.Results The sensitivity and specificity of ELISA were 70.6% and 91.6% respectively,with the positive predictive value of serological test as 82.2%,and the negative predictive value as 84.9%,based on results of IFA.The positive rate was 34.5% among 357 healthy people on indirect ELISA but was 35.6% among 239 people with IFA.Conclusion The results indicated that the indirect ELISA was a very quick, sensitive and available method for detecting Bartonella henselae in human beings,as well as a high positive percent age of Bartonella henselae among the healthy people of Changping Beijing. |
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