| 邵祝军,任红宇,徐丽,刁保卫,李伟,阚飙,李马超,高源,崔志刚,徐建国,梁晓峰,李艺星,刘丹青,杨梦,张铁钢,李漫时.中国C群脑膜炎奈瑟菌脉冲场凝胶电泳分析[J].Chinese journal of Epidemiology,2007,28(8):756-760 |
| 中国C群脑膜炎奈瑟菌脉冲场凝胶电泳分析 |
| Molecular typing of Neisseria meningitidis serogroup C strains with pulsed field gel electrophoresis in China |
| Received:April 10, 2007 Revised:August 10, 2007 |
| DOI： |
| KeyWord: 脑膜炎奈瑟菌 C群菌株 脉冲场凝胶电泳 分子分型 |
| English Key Word: Neisseria meningitidis Serogroup C Pulsed field gel electrophoresis Molecular typing |
| FundProject:国家"十五"科技攻关计划资助项目(2005BA711A09) |
| Author Name | Affiliation | E-mail | | SHAO Zhu-jun | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | REN Hong-yu | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | XU Li | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | DIAO Bao-wei | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | LI Wei | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | KAN Biao | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | LI Ma-chao | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | GAO Yuan | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | CUI Zhi-gang | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | LIANG Xiao-feng | National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | xujg@public.bta.net.cn | | LI Yi-xing | 中国疾病预防控制中心免疫规划中心 | | | LIU Dan-qing | 中国疾病预防控制中心免疫规划中心 | | | YANG Meng | 安徽省疾病预防控制中心 | | | ZHANG Tie-gang | 江西省疾病预防控制中心 | | | LI Man-shi | 北京市疾病预防控制中心 | | | XU Jian-guo | 山东省疾病预防控制中心 | |
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| Abstract: |
| 目的对中国C群脑膜炎奈瑟菌分离株进行脉冲场凝胶电泳(PFGE)分析,了解安徽省C群流行性脑脊髓膜炎(流脑)暴发菌株及国内C群脑膜炎奈瑟菌菌株的分子流行病学特征.方法 212株C群脑膜炎奈瑟菌菌株分离自流脑患者、密切接触者和健康人群鼻咽部,包括安徽省C群流脑菌株48株,其中38株与C群流脑暴发相关.脉冲场凝胶电泳选用NheⅠ限制性内切酶,聚类分析选用BioNumerics软件.结果 212株C群脑膜炎奈瑟菌菌株共分为43个PFGE带型,命名为AH1～AH43,AH1是中国C群脑膜炎奈瑟菌主要的带型,占69.3%(n=147),分布于11个省市.安徽省48株C群菌株共分为3个PFGE型别(AH1、AH2、AH3),45株(93.8%)为AH1型.安徽省38株与C群流脑暴发相关的菌株中,37株(97.4%)为AH1型.全国53株流脑患者分离菌株中,AH1型菌株占67.9%(36/53);121株流脑病例密切接触者分离菌株中,AH1型占71.9%(87/121),38株健康人群鼻咽部分离菌株中,AH1型占63.2%(24/38).结论 AH1型C群脑膜炎奈瑟菌是中国目前C群流脑流行的主要克隆群,安徽省C群流脑暴发是由AH1型C群脑膜炎奈瑟菌引起的,已在全国呈扩散流行的趋势. |
| English Abstract: |
| Objective To study the characteristics of epidemiology and molecular typing on Neisseria meningitidis serogroup C strains associated with outbreaks of Anhui province and sporadic cases in China,using pulsed field gel electrophoresis(PFGE).Methods 212 Neisseria meningitidis serogroup C strains were isolated from invasive meningococcal cases,close contacts and healthy carriers,including 48 strains from Anhui province with 38 strains associated with serogroup C outbreaks.PFGE were performed by genomic DNA digestion with NheⅠrestriction enzyme.The results of PFGE were analyzed by BioNumerics software(Version 4.0,Applied Maths BVBA,Belgium).Results A total number of 212 Neisseria meningitidis serogroup C isolates were typed by 43 patterns,named AH1 to AH43.In China, AH1 pattern was the major PFGE pattern with 69.3%(n=147)of all strains,distributed in 11 provinces.Three types of PFGE patterns(AH1 to AH3)were found in 48 strains from Anhui province,in which,93.8%(n=45)belonged to AH1.97.4%(n=37)of 38 strains associated with serogroup C outbreaks in Anhui province showed AH1 pattern.A total of 53 serogroup C strains were isolated from invasive meningococcal cases with 67.9%(36/53)of AH pattern.71.9%(87/121)of serogroup C strains isolated from contacts of invasive meningococcal cases was AH1 pattern and 63.2%(24/38)of the strains from healthy carriers showed AH1 pattern.Conclusion By PFGE typing and analysis,AH1 pattern of Neisseria meningitidis serogroup C strains was proved to be the main clone which causing the outbreaks in Anhui province and might be responsible for the sporadic serogroup C meningococcal disease epidemics else where in the country. |
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