魏绍振,杨汉青,郑谊,于晓涛,慕有,于守鸿,谢辉,宁刚,金星,李存香,冯建萍,王祖郧.辣根过氧化物酶结合鼠疫F1单克隆抗体免疫染色法快速检测鼠疫菌的特异性试验[J].Chinese journal of Epidemiology,2007,28(8):830 |
辣根过氧化物酶结合鼠疫F1单克隆抗体免疫染色法快速检测鼠疫菌的特异性试验 |
Specificity experiment on rapid detection of Yersinia pestis with HRP-F1McAb inmunostaining method |
Received:January 05, 2007 Revised:August 10, 2007 |
DOI: |
KeyWord: 鼠疫菌 免疫染色法 |
English Key Word: Yersinia pestis Inmunostaining method |
FundProject:国家"十五"重点攻关课题资助项目(20047BA718B07);青海省疾病预防控制中心资助项目 |
Author Name | Affiliation | E-mail | WEI Shao-zhen | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | YANG Han-qing | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | ZHENG Yi | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | YU Xiao-tao | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | MU You | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | YU Shou-hong | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | XIE Hui | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | NING Gang | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | JIN Xing | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | LI Cun-xiang | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | FENG Jian-ping | Institute for Endemic Disease Prevention and Control, Xining 811602, China | | WANG Zu-yun | Institute for Endemic Disease Prevention and Control, Xining 811602, China | qhedpc@vip.com |
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Abstract: |
目前,对鼠疫菌的形态检查及搜寻均依靠非特异性的染色法,而鼠疫菌的形态在很多种情况下具有多变性,两极浓染的革兰阴性菌亦非鼠疫菌所特有的特征.因此,易造成假阳性而致误诊.辣根过氧化物酶结合鼠疫F1单克隆抗体(HRP-F1McAb)免疫染色法解决了上述弊端. …… |
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