| 刘春艳,闫静,刘亚谊,谢正德.EB病毒相关性传染性单核细胞增多症的血清学诊断[J].Chinese journal of Epidemiology,2007,28(9):898-900 |
| EB病毒相关性传染性单核细胞增多症的血清学诊断 |
| Study on the serological diagnosis of Epstein-Barr virus among patients with mononucleosis |
| Received:March 26, 2007 Revised:September 10, 2007 |
| DOI： |
| KeyWord: EB病毒 传染性单核细胞增多症 血清学 抗体亲和力 |
| English Key Word: Epstein-Barr virus Infectious mononucleosis Serology Antibody avidity |
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| Abstract: |
| 目的探讨EB病毒(EBV)抗体检测在诊断EBV相关性传染性单核细胞增多症(IM)中的意义。方法北京儿童医院2005年1月至2006年12月住院治疗诊断为IM的患儿220例,人院后采集单份血清标本,采用间接免疫荧光法检测抗EBV-CA-IgM抗体、抗EBV-CA-IgG抗体、抗EBV-EA-IgG抗体、抗EBV-NA-IgG抗体及EBV-CA-IgG抗体亲和力。结果220例诊断IM患儿中,各种抗EBV抗体的阳性率分别为:抗EBV-CA-IgG 100%,抗EBV-CA-IgM 95.9%,抗EBV-EA-IgG 79.5%,抗EBV-NA-IgG 4.1%。抗EBV-CA-IgG抗体为低亲和力的患儿204例(92.7%)。186例患儿(84.6%)EBV抗体种类表现为EBV-CA-IgM和EBV-CA-IgG均阳性,EBV-NA-IgG阴性且EBV- CA-IgG为低亲和力抗体,是IM病例中抗EBV抗体反应的主要类型。结论4种抗EBV抗体(CA-IgG、CA-IgM、EA-IgG、NA-IgG及CA-IgG亲和力)检测结果能够为临床提供更多信息,从而更加准确地判断EBV感染的时期,对于IM诊断提供更可靠的确诊依据。 |
| English Abstract: |
| Objective To explore the relationship of Epstein-Barr virus(EBV)specific antibodies detection and the diagnoses of infectious mononucleosis(IM)caused by EBV.Methods Single serum samples were collected from 220 inpatients with the diagnosis of IM between January 2005 and December 2006 in Beijing Children's hospital.The samples were detected for EBV-CA-IgM,EBV-CA-IgG,EBV-EA- IgG,EBV-NA-IgG and the avidity of EBV-CA-IgG by indirect immunofluorescent assay.Results The positive rate of EBV-CA-IgG,EBV-CA-IgM,EBV-EA-IgG and EBV-NA-IgG were 100 %,95.9 %,79.5 % and 4.1% respectively.Low-avidity EBV-CA-IgG was detected in 204 patients(92.7%)with positive EBV-CA-IgM and EBV-CA-IgG,negative EBV-NA-IgG,low-avidity EBV-CA-IgG were the main pattern of EBV antibody in IM patients(84.6 %).Conclusion The existence of EBV specific antibody(CA-IgG, CA-IgM,EA-IgG,NA-IgG and avidity of CA-IgG)could add more information to identify the stage of EBV infection so as to provide more reliable serological evidence for the diagnosis of IM. |
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