江苏省宜兴地区丙型肝炎病毒F蛋白抗体检测

邓小昭; 蒋春梅; 许可; 王忠灿; 丁伟良; 喻荣彬; 王洁; 吴超; 张云

Abstract

邓小昭,蒋春梅,许可,王忠灿,丁伟良,喻荣彬,王洁,吴超,张云.江苏省宜兴地区丙型肝炎病毒F蛋白抗体检测[J].Chinese journal of Epidemiology,2007,28(11):1116-1118

江苏省宜兴地区丙型肝炎病毒F蛋白抗体检测

Detection of serum anti-F antibody in hepatitis C virus infected patients

Received:June 15, 2007

DOI：

KeyWord: 丙型肝炎病毒 F蛋白检测

English Key Word: Hepatitis C virus F protein Detection

FundProject:江苏省高校自然科学研究指导性计划基金资助项目(03KJD330144)；江苏省自然科学基金资助项目(BK2004011)

Author Name	Affiliation	E-mail
DENGXiao-zhao	Medicial Institute of Nanjing Amry, Nanjing 210002, China
JIANG Chun-mei	南京医科大学公共卫生学院流行病与统计学系
XU Ke	南京医科大学公共卫生学院流行病与统计学系
WANG Zhong-can	Medicial Institute of Nanjing Amry, Nanjing 210002, China
DING Wei-liang	宜兴市第一人民医院
YU Rongbin	南京医科大学公共卫生学院流行病与统计学系
WANG Jie	南京医科大学公共卫生学院流行病与统计学系
WU Chao	南京大学医学院附属鼓楼医院
ZHANG Yun	Medicial Institute of Nanjing Amry, Nanjing 210002, China	zhangyunlll@sohucom

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Abstract:

目的检测江苏省宜兴地区丙型肝炎(丙肝)患者血清丙肝病毒(HcV)一F抗体及其分布。方法利用基因重组获得的HCV-F／GST蛋白作为抗原，包被酶联反应板，ELIsA间接法检测120例丙肝患者、1，例乙肝患者、3例戊肝患者及10份正常人血清HCv-F抗体，结合丙肝患者的临床资料及疾病特征，统计分析HCV-F抗体的分布情况及与HcV感染的关系。结果120例丙肝患者血清HCV-F抗体阳性82例，阳性率68％，而15例乙肝、3例戊肝患者及10份正常人血清标本均未检出阳性；资料分析显示HCV-F抗体阳性率与HCV患者年龄、临床类型之问差异有统计学意义，51岁以上年龄组的HCV-F抗体阳性率是20～50岁组的6.675倍(95％cJ：2.407～19.071)，并且随着病程进展，阳性率也随之增高(0R=2.749，95％C，：1.470～5.141)。结论江苏省宜兴地区Hcv感染者血清中存在HCV-F抗体，并可能与病程相关。

English Abstract:

objective To assess the prevalence of serum anti-F in patients with hepatitis C virus(HCV)infection and the distribution of anti-F.methods The recombinant protein(HCv-F／GST)wascoated onto micro titer plates as antigen.Sera of 120 patients with hepatitis C virus infection，1 5 patientswith hepatitis B，3 patients with hepatitis E and 10 normal sera were tested by indirect ELISA for detectingant0F. results 82 samples out of the 120(68％)HCV infected patients exhibited a positive anti-Freaction，showing significant difference from the contrOls with no HCV infection(P<0.O 1).Data fromlogistic analysis showed that the positive rate of anti-F was higher in patients over 50 year olds(oR=6.675，95％CJ：2.407-19.071).Patients of midrange，severe phase and hepatic cirr}10sis had higher ratethan the others(OR=2.749，95％CJ：1.470-5.141).conclusion Prevalence and distribution of anti-F inYixing hepatitis C patients was rep。rted and which might be related to the progression of HCV infection.

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