| 周诚,黄维金,吴星,蓝海云,辜文洁,黄果勇,张华远,祁自柏,李河民.戊型肝炎病毒感染动物模型应用于抗-HEV试剂评价的初步研究[J].Chinese journal of Epidemiology,2008,29(1):48-51 |
| 戊型肝炎病毒感染动物模型应用于抗-HEV试剂评价的初步研究 |
| Primary evaluation of anti-HEV diagnostic reagent by experimental infection animal model with hepatitis E virus |
| Received:May 17, 2007 Revised:June 18, 2012 |
| DOI： |
| KeyWord: 戊型肝炎病毒 动物模型 酶联免疫吸附试验 |
| English Key Word: Norovirus Rotavirus Infants and young children Acute diarrhea |
| FundProject:国家自然科学基金资助项目(30270067) |
| Author Name | Affiliation | E-mail | | ZHOU Cheng | 中国药品生物制品检定所,北京,100050 | yqianbjc@263.net | | HUANG Wei-jin | 中国药品生物制品检定所,北京,100050 | | | WU Xing | 中国药品生物制品检定所,北京,100050 | | | LAN Hai-yun | 中国药品生物制品检定所,北京,100050 | | | GU Wen-jie | 中国药品生物制品检定所,北京,100050 | | | HUANG Guo-yong | 中国药品生物制品检定所,北京,100050 | | | ZHANG Hua-yuan | 中国药品生物制品检定所,北京,100050 | | | QI Zi-bai | 中国药品生物制品检定所,北京,100050 | | | LI He-min | 广西壮族自治区疾病预防控制中心 | |
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| Abstract: |
| 目的 应用实验室戊型肝炎病毒(HEV)感染恒河猴动物模型对抗-HEV诊断试剂进行初步的评价.方法 用1和4基因型的HEV静脉注射分别感染4只恒河猴，检测实验猴的丙氨酸氨基转移酶(ALT)水平，逆转录聚合酶链反应(RT-PCR)方法检测粪便标本中HEV RNA，抗-HEV IgG试剂(GL-IgG，WT-IgG)分别检测实验猴系列血清抗体水平.结果 HEV感染的8只实验猴均出现粪便排毒，1和4基因型HEV感染的实验猴分别有1只和2只出现ALT升高，GL-IgG试剂检测1型HEV实验感染猴有2只抗体阳转，4型HEV实验感染猴有1只抗体阳转|而WT-IgG试剂检测1、4型HEV实验感染猴的抗体均阳转.两种试剂检测感染猴窗口期抗体阳转时间相近，GL-IgG试剂抗体阳性持续到12周，WT-IgG试剂在16周观察期内均阳性.结论 两种试剂均可检测出感染实验猴的抗体，但WT-IgG试剂具有检测灵敏度较高的特点。 |
| English Abstract: |
| Objective To study the clinical manifestations for norovirus gastroenteritis in infants and young children.Methods Stool specimens were collected from infants and children with acute diarrhea who visited the affiliated Children's Hospital to Capital Institute of Pediatrics from January 2002 to December 2006.Enzyme-linked immunosorbent assay (ELISA) was used to detect human norovirus antigen in stool specimens and polyacrylamide gel electrophoresis (PAGE) was performed to detect rotavirus genome.Results Out of the 318 specimens under testing,79 showed positive for norovirus antigen,with a positive rate of 24.8% (79/318).Among those positive specimens,48(48/79,60.8%) were detected in October to December,suggesting the seasonal preference of the virus.Most of the positive specimens (91.2%) were from those under 2 years of age.Rotavirus genome were detected from 16 out of 79 norovirus positive specimens (16/79,20.3 %),indicating those patients were co-infected by these two viruses.There was significant difference found in the severity of fever but not in the frequencies of diarrhea between rotavirus and norovirus co-infection group and noroviral infection group.Fourteen out of 79 norovirus positive patients were admitted to hospitals under the diagnosis other than gastroenteritis but started to develop symptoms of diarrhea between 1 to 11 days after hospitalization.Conclusion Norovirus seemed one of the most important pathogens for acute diarrhea among infants and young children and could cause nosocomial infectious gastroenteritis. |
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