| 鞠爱萍,王长军,李明,程功,郑峰,潘秀珍,陆承平,唐家琪.猪链球菌2型反应调控因子RevS突变株的构建[J].Chinese journal of Epidemiology,2008,29(1):59-64 |
| 猪链球菌2型反应调控因子RevS突变株的构建 |
| Construction of RevS gene knock-out mutant of Streptococcus suis serotype 2 |
| Received:August 30, 2007 Revised:June 05, 2012 |
| DOI： |
| KeyWord: 猪链球菌2型 反应调控因子 基因敲除 致病性 |
| English Key Word: Bartonella henselae Direct enzyme linked immunosorbent assay |
| FundProject:国家自然科学基金资助项目(30371246) |
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| Abstract: |
| 目的 构建猪链球菌2型强毒株05ZYH33反应调控因子RevS基因敲除突变体，研究基因敲除后对细菌基本生物学性状及对小鼠和猪的致病性的影响.方法 构建中间为壮观霉素抗性基因，两侧为RevS编码基因上、下游同源序列的基因敲除载体，通过同源重组和PCR法鉴定，获得Revs编码基因完全被壮观霉素抗性基因替代的突变株.体外观察基因敲除后细菌的稳定性、生长曲线、菌落形态、溶血性、染色情况、镜下形态及蛋白表达等基本生物学性状有无发生明显改变.以108CFU野毒株和缺失株分别感染BALB/c小鼠和20日龄仔猪，观察致病性有无明显区别.结果 PCR分析显示RevS编码基因完全被壮观霉素抗性基因替代，基因敲除后细菌的基本生物学性状无明显改变.动物感染实验显示，RevS缺失株对小鼠的致病性显著减弱，但对其主要宿主猪的致病性未见显著改变.结论 成功构建了猪链球菌2型强毒株05ZYH33反应调控因子RevS基因敲除突变株，基因敲除后未明显影响细菌的基本生物学性状，但对小鼠和猪的致病性有所减弱。 |
| English Abstract: |
| Objective To detect Bartonella henselae IgG antibody among healthy people in Changping,Beijing.Methods Using indirect enzyme linked immunosorbent assay (ELISA) and immunofluorescence antibody assay(IFA) to detect IgG antibody of Bartonella henselae among human beings.Results The sensitivity and specificity of ELISA were 70.6% and 91.6% respectively,with the positive predictive value of serological test as 82.2%,and the negative predictive value as 84.9%,based on results of IFA.The positive rate was 34.5% among 357 healthy people on indirect ELISA but was 35.6% among 239 people with IFA.Conclusion The results indicated that the indirect ELISA was a very quick, sensitive and available method for detecting Bartonella henselae in human beings,as well as a high positive percent age of Bartonella henselae among the healthy people of Changping Beijing. |
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