胞浆型磷脂酶A2家族基因多态性与精神分裂症的关联性研究

俞琼; 史杰萍; 寇长贵; 孟祥飞; 于雅琴

Abstract

俞琼,史杰萍,寇长贵,孟祥飞,于雅琴.胞浆型磷脂酶A2家族基因多态性与精神分裂症的关联性研究[J].Chinese journal of Epidemiology,2008,29(2):173-176

胞浆型磷脂酶A2家族基因多态性与精神分裂症的关联性研究

Study on the genetic association between the polymorphism of eytosolic phospholipase A2 family genes and schizophrenia

Received:September 17, 2007 Revised:June 18, 2012

DOI：

KeyWord: 精神分裂症胞浆型磷脂酶A2 基因多态性

English Key Word: Schizophrenia Cytosolic phospholipase A2 Genotype polymorphism

FundProject:国家自然科学基金资助项目(30671808);教育部博士点基金资助项目(20040183043);吉林省科技厅医学专项课题资助项目(20050135)

Author Name	Affiliation	E-mail
YU Qiong		yuyaqin5540@163.com
SHI Jie-ping
KOU Chang-gui
MENG Xiang-fei
YU Ya-qin

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Abstract:

目的探讨中国北方汉族人群胞浆型磷脂酶A2(cPLA2)家族基因多态性与精神分裂症的遗传关联性.方法采用聚合酶链反应(PCR)和连接酶检测反应(LDR)方法,在201个精神分裂症患者核心家系中检测cPLA2家族基因上的10个单核苷酸多态性(SNPs),对结果进行单倍型相对风险分析(HRR)、传递不平衡分析(TDT)、单倍型分析和多位点联合分析.结果各位点在精神分裂症病例组和对照组中基因型分布均符合Hardy-Weinberg平衡.HRR和TDT分析表明,检测的10个SNPs位点与精神分裂症无关联性(P＞0.05).单倍型分析结果显示,由同一染色体上各位点组成的单倍型与精神分裂症均无关联性(P＞0.05).多位点联合作用分析显示,rs2162886与rsl668589,rs891014与rsl668589,rs2307279与rs7542180位点的联合作用与精神分裂症相关联(χ²=6.913,P=0.032;χ²=8.393,P=0.015;χ²=8.447,P=0.038).结论 cPLA2家族基因中存在多个与精神分裂症关联的易感位点.

English Abstract:

objective To investigate the genetic association between the polymorphism of cytosolic phospholipase A2 (cPLA2) family genes and schizophrenia in the North Han Chinese.Methods Method of polymerase chain reaction-based ligase detection reaction (PCR-LDR) was applied to genotype 10 single nucleotide polymorphisms(SNPs) of cPLA2 family genes among 201 pedigrees consisting of fathers, mothers and affected offsprings with schizophrenia.Haplotype relative risk(HRR) test, transmission disequilibrium test (TDT),haplotype transmission analysis and multiple locus analysis were conducted to analyze the genotyping data.Results The genotypic frequency of cPLA2 gene did not deviate from Hardy- Weihberg equilibrium in both case and control groups.HRR and TDT showed that the 10 SNPs were not associated with schizophrenia (P > 0.OS).Analysis for haplotype transmission showed that no haplotype systems was associated with schizophrenia (P >0.OS).Results from COA and COG tests showed a disease association for the rs2162886-rs1668589, rs891014-rs1668589 and rs2307279-rs7542180 combinations (x²=6.913,P=0.032;x²=8.393, P=0.015;x²=8.447,P=0.038).Conclusion Many loci in the cPLA2 family genes were associated with schizophrenic.

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