| 魏星,沈定霞,闫中强,罗燕萍,曹敬荣.耐碳青霉烯鲍曼不动杆菌的传播和分子特征[J].Chinese journal of Epidemiology,2008,29(3):277-281 |
| 耐碳青霉烯鲍曼不动杆菌的传播和分子特征 |
| Transmission and molecular characteristics of carbapenem-resistant Acinetobacter baumannii |
| Received:December 06, 2007 Revised:December 06, 2007 |
| DOI： |
| KeyWord: 鲍曼不动杆菌 碳青霉烯类 医院感染 |
| English Key Word: Acinetobacter baumannii Carbapenem Nosocomial infections |
| FundProject:军队"十一五"课题资助项目(06MA294) |
| Author Name | Affiliation | E-mail | | WEI Xing | Department of Microbiology, PLA General Hospital, Beijing 100853, China | | | SHEN Ding-xia | Department of Microbiology, PLA General Hospital, Beijing 100853, China | shendx301@yahoo.com | | YAN Zhong-qiang | Department of Microbiology, PLA General Hospital, Beijing 100853, China | | | LUO Yan-ping | Department of Microbiology, PLA General Hospital, Beijing 100853, China | | | CAO Jing-rong | Department of Microbiology, PLA General Hospital, Beijing 100853, China | |
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| Abstract: |
| 目的 分析多个患者多种标本中分离的耐碳青霉烯鲍曼不动杆菌的传播及分子特征.方法 用wHONET 5.4软件分析耐碳青霉烯鲍曼不动杆菌菌株的一般情况,结合患者病历复习,了解所分离菌株的传播和致病作用;利用脉冲场凝胶电泳(PFGE)分析基因型,采用多重PCR检测碳青霉烯酶基因,确定菌株的分子流行特征以及相互之间的亲缘关系.结果 从13例患者的2种或2种以上来源的标本中共获得耐碳青霉烯鲍曼不动杆菌29株.PFGE分型显示共有两种不同基因型,其中A型22株,来自11名患者;B型7株,来自4名患者.PCR扩增结果显示,除1株菌外,所有菌株均含有碳青霉烯酶基因0XA-23;除3株菌外,均含有Ⅰ类整合子基因.结论 所检测的耐碳青霉烯的鲍曼不动杆菌有2个基因型,以A型为主.绝大多数鲍曼不动杆菌含OXA-23型碳青霉烯酶基因和Ⅰ类整合子基因.该菌很容易通过患者自身传播及相互传播,引起医院内耐药菌感染流行. |
| English Abstract: |
| Objective To study the mode of transmission and molecular characteristics on carbapenem-resistant Acinetobacter baumannii strain. Strains were isolated from different parts of samples in various patients. Methods Clinical information of carbapenem-resistant Acinetobacter baumannii isolates were stored and analyzed by WHONET 5.4 software. The transmission and pathopoiesis of the strains were learned through case file review. Genotypes of isolates were identified by pulse-field gel
electrophoresis(PFGE)and genes of carbapenemase were detected by multiple PCR, in order to find molecular characteristics and relatedness between strains. Results 29 stains of Acirzetobacter baumannii resistant to carbapenem were isolated from 2 or more kinds of samples among 13 patients'. Two genotypes were identified by PFGE: genotype A was obtained from 22 isolates in 11 patients and genotype B was obtained from 7 isolates in 4 patients. PCR amplification showed that all strains possessed OXA-23 gene
except h and all strains possessed Integrasegene I except 3.Conclusion There were 2 different genotypes from 29 strains of earbapenm-resistant Acinetobacter baumannii with Genotype A as the main type. OXA- 23 carbapenmase gene and integrase gene } were detected from most of the isolates. All the strains could be easily transmitted in the body of the patients and among patients, hence becaming the epidemic pathogen of iatrogenic infection. |
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