任瑞文,徐晓立,方美玉,刘建伟,白志军,程刚锋,赵文忠.疏水性区域及稀有密码子对登革2型病毒E基因原核表达影响的分析[J].Chinese journal of Epidemiology,2008,29(5):516-517 |
疏水性区域及稀有密码子对登革2型病毒E基因原核表达影响的分析 |
Express characterization of dengue virus type 2 structural gene E |
Received:October 11, 2007 |
DOI: |
KeyWord: 登革病毒 原核表达 结构基因 |
English Key Word: Dengue virus Prokaryotic expression Structural gene E |
FundProject:广东省科技计划项目(20006836008005);全军医药卫生科研基金资助课题(06MAl29) |
Author Name | Affiliation | E-mail | Ren Ruiwen | Guangzhou Military District Center for Disease Control and Prevention, Guangzhou 510507, China | | Xu Xiaofang | Guangzhou Military District Center for Disease Control and Prevention, Guangzhou 510507, China | xuxiaoli@scau.edu.cn | Fang Meiyu | Guangzhou Military District Center for Disease Control and Prevention, Guangzhou 510507, China | | Liu Jianwei | Guangzhou Military District Center for Disease Control and Prevention, Guangzhou 510507, China | | Bai Zhijun | Guangzhou Military District Center for Disease Control and Prevention, Guangzhou 510507, China | | Chen Gangfeng | Guangzhou Military District Center for Disease Control and Prevention, Guangzhou 510507, China | | Zhao Wenzhong | 华南农业大学测试中心 | |
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Abstract: |
登革病毒E蛋白是病毒主要毒力蛋白,带有型特异及属特异抗原决定簇,既能诱导宿主产生保护性的中和抗体,又能诱导产生血凝抑制抗体,与登革出血热(DHF)和登革休克综合征(DSS)的致病机制有关[1]。有学者已经利用包括大肠埃希菌、毕赤酵母、昆虫细胞等多种表达系统对其表达条件进行了探讨,由于受未知因素的影响,登革病毒E蛋白的原核重组表达一直不理想[2~4]。本研究对登革病毒E蛋白不同区域的原核表达进行了较为系统的分析,探讨疏水性区域及稀有密码子登革病毒E蛋白表达的影响,并对部分区域进行了高效原核表达。广东省科技计划项目(20006836008005);全军医药卫生科研基金资助课题(06MA129) |
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