| 李晴,陈文,杨占秋.湖北地区HFRS患者血清与欧洲汉坦病毒重组核抗原的反应性研究[J].Chinese journal of Epidemiology,2008,29(6):577-580 |
| 湖北地区HFRS患者血清与欧洲汉坦病毒重组核抗原的反应性研究 |
| The reactivity of sera from hemorrhagic fever in patients with renal syndromes to the recombination nucleotide proteins from European hantaviruses in Hubei province |
| Received:December 27, 2007 |
| DOI：10.3321/j.issn:0254-6450.2008.06.015 |
| KeyWord: 肾综合征出血热|汉坦病毒|抗体水平 |
| English Key Word: Hemorrhagic fever with renal syndromes|Hantavirus|Antibodies'level |
| FundProject:国家“863”高技术研究发展计划资助项目(2007AA022465)；国家自然科学基金资助项目(30770096)；立陶宛EU Project BIOCEL基金资助项目；厦门大学引进人才科研启动基金资助项目(Z03111) |
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| Abstract: |
| 目的 用欧洲汉坦病毒流行株制备重组核抗原，检测湖北地区HFRS患者血清中汉坦病毒特异性抗体，观察流行于不同地区汉坦病毒的相关性．方法 收集湖北地区34例HFRS患者急性/恢复期血清，以ELISA法检测血清标本对欧洲汉坦病毒重组核抗原(rNP)的反应性．结果 多不拉伐病毒(DOBV)-rN |
| English Abstract: |
| Objective Five yeast-expressed recombination nucleotide proteins of European hantaviruses were prepared as coated antigens to detect hantavirus-specific antibodies in sera from hemorrhagic fever with renal syndromes (HFRS) in Hubei province, through ELISA assay. The relativity among hantaviruses prevailing in different areas was investigated. Methods 34 pairs of acute/convalescent serum samples were collected from HFRS patients in Hubei during 1985 - 1989 and 1996-2000.ELISA assay was performed to detect the reactivity of these sera to different hantavirus-recombinant nucleocapsid proteins(HV-rNP) which were derived from puumala virus (PUUV), dobrava virus (DOBV) while using hantaan virus (HTNV) to serve as control. Qualitative results were used to analyze the detection rate and the quantitative results of optical density values were used to investigate the antibodies' level and the changes. Results The detective efficiency of rNP against IgG antibody in samples was as follows:HTNV-rNP＞DOBV-rNP＞PUUV-rNP. As to the detection of IgA, it was: DOBV-rNP＞HTNV-rNP＞PUUV-rNP. However, there was no difference between DOBV-rNP and HTNV-rNP when the hantavirus-specific IgM was detected. PUUV-rNPshowed a very weak reactivity to all the antibodies in samples, but 3-pair samples reacted strongly to all the three subtype-rNP of PUUV. Results from quantitative analysis revealed that there was a relative higher level of IgM and IgA in acute phase sera. No significant difference between IgM and IgA levels was found and the level of IgG was low. A high level of IgA was detected in convalescent sera. Moreover, the level of IgA and IgG significantly increased with the progress of the disease. Conclusion DOBV-rNP had a high detective efficiency to serum samples from HFRS patients in Hubei. HV-specific IgA was kept on a high level in acute and convalescent phases and had important implications for the surveillance of HFRS. Also,it is assumed that PUUV and DOBV might have existed in Hubei province. |
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