| 王憨杰,段松,项丽芬,杨跃诚,涂源泉,王继宝,姚均,齐军,蒋岩.云南省德宏州HIV-1新近感染者基因型耐药分析[J].Chinese journal of Epidemiology,2008,29(9):905-908 |
| 云南省德宏州HIV-1新近感染者基因型耐药分析 |
| Study on the drug resistance situation among recently infected HIV-I patients in Dehong |
| Received:April 23, 2008 |
| DOI： |
| KeyWord: 艾滋病病毒|新近感染者|基因型耐药 |
| English Key Word: Human immunodeficiency virus|New recent infections|Genotypie resistance |
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| Abstract: |
| 目的了解云南省德宏州HIV-1新近感染者中耐药的发生情况。方法 收集德宏州2006年7-12月期间所有的HIV-1阳性样品共1048份,使用BED捕获酶免疫试验进行新近感染检测,共检出新近感染样品101份;使用Bayer公司的TruGene方法进行基因型耐药检测,应用所有样品的序列构建系统进化树以确定样品的亚型。结果云南省德宏州HIV.1新近感染者巾的耐药发生率为6.25%(4/64),样品PR区中M36I/V、L63P和H69K等次要耐药突变出现的频率较高,分别占81.25%、70.31%和65.63%;HIV-1流行株以C/CRF07 BC/08_BC为主,占65.63%(42/64)。结论德宏州HIV-1新近感染者中耐药的流行处于中等水平;有必要加强HIV-1的耐药监测。 |
| English Abstract: |
| Objective To study the HIV-1 drug resistance (DR) situation among newly infected persons in Dehong.Methods 1048 HIV-1 positive blood samples from July to December in 2006 from Dehong prefecture of Yunnan,were collected.HIV drug resistance were tested using TruGene in newly infected people that were distinguished with BED-CEIA,while the subtype were determined with phylogenetic analysis using a set of reference sequences available on the Los Alamos Database.Results Of sixty-four successfully analyzed samples,drug resistance mutations were detected in 4 samples with the resistance rate as 6.25%.Minor mutation in PR region such as M36I/V,L63P and H69K appeared frequently and the rates were 81.25%,70.31%and 65.63%respectively.The predominantly prevalent strains were seen as C/CRF07_BC/08_BC(65.63%,42/64) in this study.Conclusion The prevalence of genotypic drug resistances in HIV-1 recent infections in Dehong prefecture appeared to be at moderate level.Drug-resistance surveillance program among HIV-1 infections should be continued and strengthened. |
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