湖南省2007年狂犬病病原学监测

戴德芳; 张红; 刘运芝; 刘富强; 唐青; 李浩; 陶晓燕

Abstract

戴德芳,张红,刘运芝,刘富强,唐青,李浩,陶晓燕.湖南省2007年狂犬病病原学监测[J].Chinese journal of Epidemiology,2008,29(10):1009-1012

湖南省2007年狂犬病病原学监测

Viral surveillance on rabies in Hunan province,in 2007

Received:June 12, 2008

DOI：

KeyWord: 狂犬病病毒病原学监测

English Key Word: Rabies virus Virology Surveillance

FundProject:

Author Name	Affiliation
DAI De-fang	Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China
ZHANG Hong	Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China
LIU Yun-zhi	Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China
LIU Fu-qiang	Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China
TANG Qing	中国疾病预防控制中心病毒病预防控制所
LI Hao	中国疾病预防控制中心病毒病预防控制所
TAO Xiao-yan	中国疾病预防控制中心病毒病预防控制所

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Abstract:

目的了解湖南省狂犬病病毒的分布及来源,从病原学角度分析该省狂犬病疫情高发的原因.方法采集湖南省人间狂犬病高、中、低疫区市售家犬脑组织及疑似病例、病犬标本,用直接免疫荧光法(DFA)和RT-PCR检测狂犬病病毒,RT-PCR阳性标本进行狂犬病病毒N基因片段核苷酸序列分析. 结果外观正常犬脑组织狂犬病病毒阳性率为2.78%,23株狂犬病病毒N基因片段核苷酸序列同源性范围为88.8%～100.0%,表明主要足同义突变,病毒株在省内的分布无明显的地域性.系统发生树揭示狂犬病病毒在湖南省省内及与周边省份(贵州、湖北、广西)和其他省份(江苏、河南)间存在扩散循环传播.疑似病例的唾液、尿液、血液标本均未检出狂犬病病毒,皮肤标本检出1例阳性. 结论湖南省内普遍存在狂犬病病毒感染犬只,省内与外省的病毒分离株有高度的亲缘关系,此为疫情高发的主要原因.

English Abstract:

Objective To understand the source and distribution of rabies virus(RV)in Hunan province with viral surveillance in order to provide scientific measures for prevention and control on rabies.Methods Brain samples from healthy-looking domestic dogs were collected in the agricultural markets at the dist6cLs of high.middle,and lOW incidence rates and detected by direct Immunofluorescence assay (DFA).Positive samples would be further detected by RT-PCR and the surveillance samples were detected bv RT.PCR.The positive samples detected bv RT-PCR were sequenced with N gene.Results The infection rate of thosc healthy-looking domestic dogs with rabies virus was 2.78％in Hunan province in 2005.23 positive samples’N gene were sequenced and their similarities were 88.8％-100.0％.The results indicated that Hunan rabies virus N gene aberrance was mainly synonymous aberrance and did not CKITy obvious regional characteristics.The rabies virus were circulating among different districts in Hunan province,and the neighboring provinces such as Guizhou,Hubei，GuangxiComparison of immunity to measles between floating and local population,Jiangsu and Henan.There were no positive samples detected in salivary，blood and urine samples.There was one positive sample detected in two skin samples.Conclusion There are dogs infected with rabies virus found in Hunan province and this study showed that rabies virus detected in Hunan had a close genetic relationship with those rabies idcntified in other provinces,suggesting that study on the immunity and management of dog related rabies should be strengthened.

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