一株未知黏附基因型的尿道致病性大肠埃希菌UPEC4030株的基因分析

吴庆刚; 张敬平; 陈锦英; 郑明寰; 尤凤兴; 姜永

Abstract

吴庆刚,张敬平,陈锦英,郑明寰,尤凤兴,姜永.一株未知黏附基因型的尿道致病性大肠埃希菌UPEC4030株的基因分析[J].Chinese journal of Epidemiology,2008,29(11):1123-1127

一株未知黏附基因型的尿道致病性大肠埃希菌UPEC4030株的基因分析

Genomic analysis of an unkown pap genotype from uropathogenic Escherichia coli 4030 strain

Received:June 17, 2008

DOI：

KeyWord: 尿道致病性大肠埃希菌基因测序未知基因型

English Key Word: Uropathogenic Escherichia coli Gene sequencing Unkown genotype

FundProject:卫生部科研基金资助项目(WKJ2006-2-10)

Author Name	Affiliation
WU Qing-gang	Wuxi Center for Disease Control and Prevention, Wuzi 214023, China
ZHANG Jing-ping	Wuxi Center for Disease Control and Prevention, Wuzi 214023, China
CHEN Jin-ying	天津医科大学基础医学院
ZHENG Ming-huan	中国疾病预防控制中心传染病预防控制所
YOU Feng-xing	Wuxi Center for Disease Control and Prevention, Wuzi 214023, China
JIANG Yong	Wuxi Center for Disease Control and Prevention, Wuzi 214023, China

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Abstract:

目的比较尿道致病性大肠埃希菌UPECA030株papG和papA基因与相关序列的差异.以明确UPECA030 papA基因变异情况及与papG的组合形式,确定是否为未知基因型.方法采用基因克隆测序方法,分别对UPEC4030株papG和papA基因进行序列分析,并与相关序列进行比较.结果 UPECA030 papA由722个碱基对组成,编码192个氨基酸的多肽.与10个基因型UPEC papA核苷酸序列和推导的氨基酸序列同源性比较,分别为36.11%～77.95%和22.20%～78.34%;与papA基因分型多重PCR引物序列比较,下游分型引物在UPEC4030 papa相应位置上的序列同源性只有10.00%～66.67%,表明该菌株papA确实为未知的基因型.UPECA030菌株papG全基因片段由1100个碱基组成,编码337个氨基酸的多肽,其核苷酸及推导的氨基酸序列与Ⅱ型抛papG代表株UPEC IA2相比较,同源性分别为99.00%和99.11%,表明UPEC4030 papA属于Ⅱ型.结论 UPECA030 papA为未知的摹因型,其papG属于Ⅱ型.

English Abstract:

Objective To investigate the differences between the sequences of papA and papG of UPECA030 strain and the related genes, to better understand the genetic variation of UPEC4030 papA and its combination forms with papG so as to identify if it was a new genotype. Methods Cloning and sequencing methods were used to analyze the sequences of papG and papA of UPEC4030 strain and to compare their related sequences. Results Through sequence analysis of papA, it was revealed that there was a 722 bp gene,encoding 192 amino acid polypeptide. The overall homology of the papa genes between UPEC4030 and the standard strains of ten Ftypes were 36.11%-77.95 % and 22.20%-78.34% at nueleotide and deduced amino acid levels. Homology between the sequences of reverse primers and the corresponding sequence of UPEC4030 papA was 10.00%-66.67%. The results confirmed that UPECA030 strain contained a novel papA variant. Through sequence analysis of UPEC4030 papG, we revealed a 1100 bp gene, encoding 337 amino acid polypeptide. The homology of the papG genes between UPEC4030 and UPEC IA2, the standard strain, was 99.00 % at nucleotide level and 99.11% at deduced amino acid and UPEC4030 strain carried class I] genotype of papG. Conclusion UPEC4030 strain contained an unknown papA variant or the new genotype and carried class II genotype of papG. The pathogenic mechanism and epidemiology call for further study.

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