龚黎明,葛琼,卢亦愚,张严峻,严菊英,周敏,余昭.浙江省2006-2007年暴发性胃肠炎中诺如病毒的分子流行病学分析[J].Chinese journal of Epidemiology,2009,30(2):147-150 |
浙江省2006-2007年暴发性胃肠炎中诺如病毒的分子流行病学分析 |
Molecular epidemiology of Norovirus in outbreaks of gastroenteritis in Zhejiang from 2006 to 2007 |
Received:August 15, 2008 |
DOI: |
KeyWord: 诺如病毒 暴发性病毒性胃肠炎 分子流行病学 |
English Key Word: Norovirus Outbreaks ofgastroenteritis Molecular epidemiology |
FundProject: |
Author Name | Affiliation | GONG Li-ming | Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | GE Qiong | Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | LU Yi-yu | Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | ZHANG Yan-jun | Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | YAN Ju-ying | Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | ZHOU Min | Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | YU Zhao | Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China |
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Abstract: |
分析浙江省2006-2007年暴发性病毒性胃肠炎中诺如病毒感染的分子流行病学特征。方法收集监测期间暴发性病毒性胃肠炎疫情患者的粪便标本及相关流行病学资料。采用RT-PCR及荧光定量RT-PCR检测诺如病毒。随机选掸部分阳性标本扩增部分多聚酶区和衣壳蛋白片段,进行序列测定,结合诺如病毒I(GI)、Ⅱ(GⅡ)基因型参考株进行进化分析。结果诺如病毒感染暴发性胃肠炎共5起,发生时间均集中于9-12月,送检标本63份,诺如病毒检测阳性45份。 |
English Abstract: |
study the molecular epidemioiogical characteristics of Norovirus gastroenteritis outbreaks in Zhejiang.Methods During January 2006 and December 2007.fecal specimens of patients collected from outbreaks of acute viml gastroenteritis were tcsted for Norovirus.Epidemiological data were also collected.Noroviruses were detected by a reverse transcription polymerase chain reaction(RT-PeR)and Real.time RT-PCR.Some positive samples were randomly selected and Rrr-PCR products were sequenced.Comparing to the nucleotide sequences of norovirus genotype Ⅰ,Ⅱ reference strains from GenBank,sequence analysis was undertaken based on partial sequence of RNA dependent RNA polymerase(RdRp)and capsid protein(VPI)gene.Results 5 Outbreaks of viral gastroenteritis caused bv Norovirus were reported.A total of 63 stools were obtained from cases with acute gastroenteritis.Noroviruses alone were detected in 45 cases and the illness appeared in autumn.Phylogenetic analysis revealed that Norovirus belonged to G Ⅱ/G Ⅱ 4 type.The strains isolated from Zhejiang were almost identical on G Ⅱ/4 variants that causing epidemics in Beijing and in the Netherlands with the homology of 99.7%and 98.5%-99.O%respectively.Phylogenetic analysis revealed that the isolates were located at the same branch as the norovirus G Ⅱ/4 variants found in Beijing and Netherlands.Conclusion Norovirus iS a major cause of outbreaks of viral gastroenterifis in Zhejiang province.GenogroupⅡ/4 variants viruses were the prevalent strains. |
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