中国脑膜炎奈瑟菌中插入序列1301的分布与分子流行病学研究

庞杏林; 侯配斌; 高源; 徐丽; 任红宇; 朱兵清; 周海健; 邵祝军

Abstract

庞杏林,侯配斌,高源,徐丽,任红宇,朱兵清,周海健,邵祝军.中国脑膜炎奈瑟菌中插入序列1301的分布与分子流行病学研究[J].Chinese journal of Epidemiology,2009,30(5):475-480

中国脑膜炎奈瑟菌中插入序列1301的分布与分子流行病学研究

Distribution and molecular epidemiologic characterics of insertion sequence IS1301 in Neisseria meningitidis isolated from China

Received:February 09, 2009

DOI：

KeyWord: 脑膜炎奈瑟菌插入序列IS1301 脉冲场凝胶电泳核酸印迹杂交

English Key Word: Neisseria meningitidis Insertion sequence 1301 Pulse field gel electrophoresis Southern blot

FundProject:

Author Name	Affiliation	E-mail
PANG Xing-lin	Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China
HOU Pei-bin	山东省疾病预防控制中心
GAO Yuan	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
XU Li	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
REN Hong-yu	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
ZHU Bing-qing	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
ZHOU Hai-jian	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
SHAO Zhu-jun	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室	shaozhujun@icdc.cn

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Abstract:

目的研究中国脑膜炎奈瑟菌(Nm)中插入序列IS1301的分布与分子流行病学特征。方法设计IS1301引物，应用PCR方法检测2007年1月至2008年10月收集全国16个省、市、自治区219株Nm菌株的IS1301，对IS1301扩增产物进行DNA测序和序列比对，PCR扩增IS1301阳性菌株进行脉冲场凝胶电泳(PFGE)分析及核酸印迹杂交，研究不同Nm菌株中IS1301的分布特征。结果219株Nm菌株中，34株携带IS1301(15。53%)，A、B、C群和不可分群的Nm菌株IS1301阳性率分别为11。11%、20。75%、6。17%和28。57%;IS1301扩增产物测序结果与GenBank登记编号ZA9092。1的Nm菌株序列相似度为94%～100%，进化树分析可分为两簇;C群Nm菌株与其他血清群菌株IS1301序列存在较大差别;研究的Nm菌株基因组中至少存在6～17个IS1301拷贝，相同PFGE带型Nm菌株所携带的IS1301拷贝数具有多态性特征。结论IS1301在中国Nm菌株中的分布具有遗传多态性。

English Abstract:

Objective To research the distribution and molecular epidemiology of insertion sequence IS1301 in Neisseria (N.) meningitidis strains in China, so as to provide scientific and available evidence for a new method of genotyping in N.meningitidis strains with IS1301.Methods Examined the IS1301 by PCR in 219 N.meningitidis strains from 16 provinces and 3 cities during 2007 and 2008 in China, productions of amplification were sent for sequencing. The positive N.meningitidis strains were analyzed by pulse field gel electrophoresis (PFGE) and nucleic acid blotting hybridization(Southern blot) by electrophoresis.Results The positive rates with IS1301 were 15.53%, 11.11%, 20.75%, 6.17% and 28.57% for four serotypes (A, B, C, N) respectively. The sequence comparability between the amplification productions and No.Z49092.1 N.meningitidis which registered in GenBank was 94%-100%. There were two types of clusters devided by cladogram analysis. There appeared large IS1301 sequence difference between the serotype C and others. The number of IS1301 replica ranged from 6-17 per strain at least. The number of IS1301 replica changed in the same type of PFGE N.meningitidis respectively.Conclusion Typing by IS1301 combined with PFGE could comprehend the homology and genetic polymorphism of N.meningitidis epidemic strains at the molecular level.

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