| 汪霞,徐葛林,吴杰,罗静,朱家鸿,周剑军,施南峰,唐青,张永振,唐建蓉,吴泰才,明贺田,沈蕊华,陈焕春.细胞培养法检测和分离狂犬病街毒的可行性研究[J].Chinese journal of Epidemiology,2009,30(6):611-615 |
| 细胞培养法检测和分离狂犬病街毒的可行性研究 |
| Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus |
| Received:February 16, 2009 |
| DOI： |
| KeyWord: 狂犬病街毒 细胞培养法 检测 |
| English Key Word: Street rabies virus Cell-culture inoculation test Detection |
| FundProject:广东省科技计划(20006B36008005);全军医药卫生科研基金(06MA129) |
| Author Name | Affiliation | E-mail | | WANG Xia | Wuhan Institute of Biological Products, Wuhan 430060, China | | | XU Ge-lin | Wuhan Institute of Biological Products, Wuhan 430060, China | xugelin@yahoo.com.cn | | WU Jie | Wuhan Institute of Biological Products, Wuhan 430060, China | | | LUO Jing | Wuhan Institute of Biological Products, Wuhan 430060, China | | | ZHU Jia-hong | Wuhan Institute of Biological Products, Wuhan 430060, China | | | ZHOU Jian-jun | Wuhan Institute of Biological Products, Wuhan 430060, China | | | SHI Nan-feng | 浙江省慈溪市疾病预防控制中心 | | | TANG Qing | 中国疾病预防控制中心病毒病预防控制所 | | | ZHANG Yong-zhen | 传染病预防控制所 | | | TANG Jian-rong | 中国药品生物制品检定所 | | | WU Tai-cai | 广西壮族自治区疾病预防控制中心 | | | MING He-tian | 安徽省阜阳市疾病预防控制中心 | | | SHEN Rui-hua | 上海市疾病预防控制中心 | | | CHEN Huan-chun | 华中农业大学兽医学院 | |
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| Abstract: |
| 目的 研究MNA细胞培养法检测和分离狂犬病街毒的可行性.方法 用MNA细胞培养法、荧光抗体实验(FAT)和双抗体夹心ELISA方法分别检测33份狂犬病街毒样品、20份狂犬病毒阴性犬脑样品和4份正常鼠脑样品.结果 MNA细胞培养法检测57份待检样品,培养48 h的实验结果显示,33份狂犬病街毒样品均为阳性,狂犬病毒阴性犬脑样品和正常鼠脑样品均为阴性,与FAT、双抗体夹心ELISA方法的检测结果完全一致.结论 MNA细胞培养法对样品中狂犬病街毒的检测具有较高的灵敏度和特异性,可用于狂犬病街毒的检测和分离. |
| English Abstract: |
| Objective Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus. Methods Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains. Results 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%. Conclusion MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus. |
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