河南省手足口病例肠道病毒71型分离株的全基因组序列分析

张绍丽; 许汴利; 郭万申; 陈立; 卫海燕; 杜燕华; 李幸乐

Abstract

张绍丽,许汴利,郭万申,陈立,卫海燕,杜燕华,李幸乐.河南省手足口病例肠道病毒71型分离株的全基因组序列分析[J].Chinese journal of Epidemiology,2009,30(9):938-941

河南省手足口病例肠道病毒71型分离株的全基因组序列分析

Complete genome sequencing of enterovirus 7l(EV71)HENAN08 strain isolated in Henan provinee

Received:April 22, 2009 Revised:July 19, 2007

DOI：

KeyWord: 肠道病毒7l型全基因组序列分析

English Key Word: nterovirus 71 Complete genome Analysis ofgenome sequence

FundProject:浙江省医药卫生科学研究基金资助项目(2004A018)

Author Name	Affiliation
ZHANG Shao1i	Collage of public Health, Zhenmhou University. Zhengzhou 450001, China
XU Bian1i	河南省疾病预防控制中心
GUO Wanshen	河南省疾病预防控制中心
CHEN Li	中国疾病预防控制中心
WEl Haiyan	河南省疾病预防控制中心
DU Yanhua	河南省疾病预防控制中心
LI Xing1e	河南省疾病预防控制中心

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Abstract:

目的对2008年河南省手足口病患者体内分离的肠道病毒71型(Ev71)进行全基冈组核苷酸序列测定。方法采用RT-PCR扩增覆盖全基因组的8个重叠基因片段，双向测序，DNAStar软件中的MegAlign等软件进行同源性分析，MEGA3软件进行进化树分析和做图。结果8个重叠基因片段序列拼接得到EV71全基冈组序列，共7405个核苷酸，与其他EV71病毒基因组构成相似，在非编码区有核苷酸的插入和缺失。同源性分析：在P1区，HENAN08株与安徽株(AnhuiFY08)、深圳株(SHZH98和SHZH03)、浙江株(Zhejian908)等毒株的核苷酸和氨基酸同源性分别大于91％和97％；与标准株(BrCr)和台湾流行株(TW2086)的核苷酸和氨基酸同源性分别大于75％和82％；与CoxsackievirusAl6(Cox．A16)的同源性最低，为68．7％和79．6％。在P2和P3区，HENAN08株与其他国内EV71株的同源性均较高，而与BrCr以及TW2086的同源性低于Cox．A16。Pi区遗传进化分析表明：HENAN08株与AnhuiFY08和Zhejian908的进化关系都密切，与Cox．A16疏远。结论HENAN08属EV71病毒的C4亚型，与AnhuiFY08、Zhejian908和SHZH03进化途径类同。

English Abstract:

objective To sequence the whole-genome of enterovirus 71(EV71)srtainisolated from patient with hand．foot and mouth in Henan province in 2008．Methods Eightoverlapping clones covering the whole viral genome were obrained by RT-PCR and the sequenceswere determined by Sanger dideoxg．mediated chain termination method．Results Data it showed that the full length of enterovirus 7 l(EV71)HENAN08 genome(not including Poly Atail)iS 7405 bp．NO deletion or insertion was detected in the coding region．There were several deletions and insertions in 5’UTR and 3’UTR regions．In Pl region．HENAN08 strain shared high homology with AnhuiFY08 strain，Zhejian908 strain and SHZH strains(SHZH98．SHzH03)but lOW homology with COX．A16．In P2 and P3 regions．HENAN08 strain shared higher nucleotide homology with COX．A16(81．7％and 83．7％)than that with BrCr and TW2086 strains．The phylogenetic analysis based on PI region demonstrates that HENAN08 strain had the nearest genetic relationship with AnhuiFY and Zhejiang strains(isolated in 2008)．Conclusion The HENAN08 strain might belong to the same genogroup with AnhuiFY08 and Zhejian908 strains as C4 gene subtypes．

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