| 展群岭,张英英,徐呜明,胡永波,吴秀玲,余建平,陈晓,朱丹,杨德雨,谢鹏.新疆伊犁地区不同品种犬博尔纳病病毒自然感染调查[J].Chinese journal of Epidemiology,2009,30(10):993-997 |
| 新疆伊犁地区不同品种犬博尔纳病病毒自然感染调查 |
| Epidemiological investigation on natural infection of different canine breeds with Borna disease virus in lH。China |
| Received:April 23, 2009 |
| DOI： |
| KeyWord: 博尔纳病病毒 磷蛋白RNA 巢式反转录一聚合酶链反应 TaqMan探针 犬 |
| English Key Word: Boma disease virus p24 RNA Nested reverse transcription—polymerase chain reaction TaqMan probe Canine |
| FundProject:“十一五”国家高技术研究发展计划(863计划)项月 (2006AA022196) |
| Author Name | Affiliation | E-mail | | ZHAN Qun-ling | ChongqingMedical University, Chongqing 400016, China | | | ZHANG Ying-ying | ChongqingMedical University, Chongqing 400016, China | | | XU Ming-ming | ChongqingMedical University, Chongqing 400016, China | | | HU Yong-bo | ChongqingMedical University, Chongqing 400016, China | | | WU Xiu-ling | First Affeliated Hospital | | | YU Jian-ping | ChongqingMedical University, Chongqing 400016, China | | | CHEN Xiao | ChongqingMedical University, Chongqing 400016, China | | | ZHU Dan | ChongqingMedical University, Chongqing 400016, China | | | YANG De-yu | ChongqingMedical University, Chongqing 400016, China | | | XIE Peng | 重庆医科大学附属第一医 院神经内科重庆跃科大学神经科学研究中心 | peng.xie58@gmail.com |
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| Abstract: |
| 目的调查新疆伊犁地区不同品种犬博尔纳病病毒(BDv)流行现状和分析其可能的种系来源。方法 采用改进的巢式反转录PCR(nRT-PCR)方法，检测犬外周血单核细胞(PBMCs)BDV磷蛋白(p24)RNA基冈片段。用BDV核蛋白(p40)RNA片段和质粒PMDl9标准品验i正p24阳性产物，排除可能的假阳性。验证后的阳性产物进行基因测序、同源比对、氨基酸序列和系统发生学分析。结果 8个品种150只犬中，仅哈萨克牧羊犬检出BDVp24片段，阳性率为11．0％(10／91)。基因序列与德国马He／80和德国绵羊s6病毒株同源相似度分别为99．2％和95．7％，氨基酸相似度为100％和89．3％，亲缘关系与He／80最近，其次为s6。结论新疆伊犁地区哈萨克牧羊犬可能存在BDV自然感染，其流行株与该地区伊犁马感染的He／80和引进德国美利坚奴绵羊的s6病毒株有关。 |
| English Abstract: |
| 0bjective To investigate the epidemioiogical pattern of Borna disease virus (BDV)among different canine breeds in Ili．China。and to analyze iIs potential phylogeny． Methods BDV p24 RNA fragments were detected from peripheral blood mononuclear cells (PBMCs)of canine bv modified nestedⅣr-PCR(nlmPCR)．Possible false positives were excluded by determination of both BDV D40 RNA fragments and PMD1 9 plasmid standards．Analysis Were performed on genetic sequence，homologous comparison，amino acid sequence and phylogeny after p24 positive products were validated．Results BDVp24 RNAfragments were found only in Kazakh (a shepherd dog)in 8 breeds of 150 cases and their overall positive rate was 11．0％(10／91)． Compared with the strain of He／80 from horse and that of S6 from sheep in Germany．the homologous similarities of Kazakh Tobet Was 99．2％and 95．7％．and that of amino acid as 1 00％and 89．3％。 respectively．The kinship of Kazakh Tobet Was close to He 80 and next to S6．Conclusion There Was potential natural BDV infection in Kazakh Tobet in IIi．and its endemic strain was concerned with He 80 infecting IIi horseand S6 of German Merino sheep introdueed into the region from Germany． |
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