中国宿主动物中Amur汉坦病毒M片段全基因序列分析

郭卫东; 张文义; 江佳富; 池海谊; 王文瑞; 王中元; 金鹰; 杨红; 方立群; 曹务春

Abstract

郭卫东,张文义,江佳富,池海谊,王文瑞,王中元,金鹰,杨红,方立群,曹务春.中国宿主动物中Amur汉坦病毒M片段全基因序列分析[J].Chinese journal of Epidemiology,2009,30(10):1051-1054

中国宿主动物中Amur汉坦病毒M片段全基因序列分析

Complete sequence of the M segment of Amur virus in rodent from China

Received:March 20, 2009

DOI：

KeyWord: 汉坦病毒 Amur病毒 M片段序列分析

English Key Word: Hantavirus Amur virus Msegment Sequence analysis

FundProject:1. 国家杰出青年科学基金；2. 国家自然科学基金；3. 传染病重大专项基金

Author Name	Affiliation	E-mail
GUO Wei-dong	Inner Mongolia Autonomous Region Center for Disease Control and Preuentdon, Huhehaote 010031, China
ZHANG Wen-yi	军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室
JIANG Jia-fu	军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室
CHI Hai-yi	呼和浩特市第一医院
WANG Wen-rui	Inner Mongolia Autonomous Region Center for Disease Control and Preuentdon, Huhehaote 010031, China
WANG Zhong-yuan	黑龙江省韦河林业局卫生防疫站
JIN Ying	黑龙江省韦河林业局卫生防疫站
YANG Hong	军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室
FANG Li-qun	军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室
CAO Wu-chun	军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室	Caowc@nic.bmi.ac.cn

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Abstract:

目的测定中国宿主动物中Amur病毒M片段全基因序列,以了解其分子特征.方法设计特异的PCR引物,用RT-PCR法分段扩增JilinAP06株M片段全长,PCR产物经克隆后进行序列测定,然后进行系统发育分析和重组分析.结果JilinAP06株M片段全基因序列共3615个核苷酸,A+T含量为59.3%,G+C含量为40.7%,包含1个单一的开放读码框架,其最大读码框架从41～3448,由3408个核苷酸组成,共编码1135个氨基酸.序列同源分析表明,JilinAP06株与中国的人源Amur HV株同源性为96.0%～97.8%,与韩国大林姬鼠来源的Soochong HV同源性为85.6%～86.7%,与HTNV原型株76-118同源性仅为79.5%,而与其他各型病毒间的同源性均低于79.0%.氨基酸同源分析,其氨基酸序列与中国的人源株Amur HV同源性为98.1%～98.4%,与韩国大林姬鼠来源的Soochong HV同源性为96.4%～97.0%,与HTNV原型株76-118同源性为91.7%,而与其他各型病毒间的同源性均低于92.0%.系统发育分析结果显示,JilinAP06与Amur病毒构成一个相对独立的分支.结论从中国宿主动物中获得了AmurHV的M片段全基因序列.

English Abstract:

Objective To study the complete sequence of Msegment of Amur virus in rodents and to explore their molecular characteristics. Methods Complete M segment of Amur virus in rodent from China was amplified by RT-PCR. The purified PCR product was cloned into pGEM-T Easy vector and then sequenced. Phylogenetic analysis on multiple nucleotide sequences was performed with the Tree PUZZLE and DNAStar software. Results The full-length of its M gene comprised of 3615 nucleotides with one open reading frame (ORF) including 3408 nucleotides and encoding a protein which comprised 1135 amino acids. The ORF was located at bases 41 to 3448. The phylogenetic analysis of JilinAP06 with other hantaviruses revealed that the complete sequence of M segment of JilinAP06 strain was closely related to those Amur viruses such as B78 strain, Liu strain and H5 strain were all from the patients. The complete sequence of M segment of JilinAP06 had only 79.5% identities with the nucleotide sequence of HTNV strain 76-118. Conclusion The complete sequence on M segment of Amur virus in rodent was first time identified in this country.

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