| 董慧瑾,钱渊,张又,赵林清,朱汝南,陈冬梅,刘立颖.北京地区2007-2008年G9型A组人轮状病毒VP7和VP4基因分析[J].Chinese journal of Epidemiology,2009,30(11):1179-1183 |
| 北京地区2007-2008年G9型A组人轮状病毒VP7和VP4基因分析 |
| Analysis on VP7and VP4 genes of human rotavirus G9 identified from children with diarrhea in Beijing, from 2007 to 2008 |
| Received:June 30, 2009 |
| DOI： |
| KeyWord: G9型轮状病毒 基因型 序列分析 |
| English Key Word: Rotavirus G9 Genotype Sequence analysis |
| FundProject: |
| Author Name | Affiliation | E-mail | | DONG Hui-jin | Laboratory of Virology.Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China | | | QIAN Yuan | Laboratory of Virology.Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China | yqianbjc@263.net | | ZHANG You | Laboratory of Virology.Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China | | | ZHAO Lin-qing | Laboratory of Virology.Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China | | | ZHU Ru-nan | Laboratory of Virology.Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China | | | CHEN Dong-mei | Laboratory of Virology.Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China | | | LIU Li-ying | Laboratory of Virology.Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China | |
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| Abstract: |
| 目的了解北京地区2007-2008年检测到的G9型A组人轮状病毒外壳蛋白VP7和VP4的基因特征.方法选取经过轮状病毒核酸杂交方法检测为G9型轮状病毒的12份儿童腹泻患儿的粪便标本,应用针对vP7全长基因的特异引物对进行RT-PCR扩增,对所获得的vP7全长基因进行克隆和测序,将所获得的序列与GenBank中的G9型原型病毒株和近期流行株的vP7基冈进行序列和种系进化分析|经巢式PCR对G9型的VP4进行P基因分型.结果12株G9型轮状病毒经vP7基因的序列比较分析得到确认.P基因分型结果显示北京地区近年来存在G9P[8]和G9P[6]型两种组合的轮状病毒感染.序列和种系进化分析发现北京G9型株VP7基因与世界范围内近期流行的G9型株-样都属于进化分支Ⅲ,彼此间的核苷酸和氨基酸同源性较高,而与国内最早报道的G9型T203进化关系较远,且北京G9P[8]和G9P[6]型株分别与国内近期报道的新G9P[8]和G9P[6]型株及相应的武汉G9型株vP7基因,在氨基酸位点上存在-些共同的氨基酸残基取代.结论北京地区近年存在G9P[8]和G9P[6]两种不同基因组合的G9型轮状病毒感染,需要进-步加强对G9型轮状病毒的分子流行病学监测. |
| English Abstract: |
| Objective To characterize the outer capsid protein VP7 and VP4 encoding genes of human rotavirus G9 strains detected in Beijing from 2007 to 2008.Methods Full length of vP7 genes of G9 rotaviruses from 12 fecal specimens previously detected by dot-blot hybridization assay were amplified by RT-PCR and sequenced after being cloned into T vector.The sequences of these VP7s were compared to VP7 genes of rotaviras G9 prototype strains and recently circulating strains around the world.VP4 genes of these 12 G9 strains were amplified by nested-PCR for Pgenotyping. Results Sequence analysis for the full length of VP7 genes from these 12 specimens confirmed that they were G9 rotaviruses.P genotyping for VP4 genes revealed that both P[8]G9 and P[6]G9 were circulating in Beijing in the last 2 years.Sequence and phylogenetic analysis demonstrated that VP7 genes of G9 strains from Bering in tllis study were clustered in the lineage Ⅲ which resembled the G9 strains circulating in other places around the world.indicated by high identities of nucleotide and deduced amino acid sequences and were distant with the fast reported G9 strain T203 identified in China in 1994.It was found that there were some consistent amino acid substitutes at the corresponding positions among VP7s from these l2 specimens and from Xinjiang and wIlIlan.botll ill G9P[8]and G9P[6] strains. Conclusion The rotavirus G9 strains both in combination of G9P[8]and G9P[6]were tireulating in Beijing in the past years.It seemed that rotavirus G9 should be included in the list of surveillance for rotavirus in China. |
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