北京市郊区八种动物戊型肝炎病毒抗体流行率调查及病毒基因型研究

耿家宝; 付红伟; 王玲; 王晓娟; 关建民; 常义宾; 李凌君; 朱永红; 庄辉; 刘全红; 彭兴春

Abstract

耿家宝,付红伟,王玲,王晓娟,关建民,常义宾,李凌君,朱永红,庄辉,刘全红,彭兴春.北京市郊区八种动物戊型肝炎病毒抗体流行率调查及病毒基因型研究[J].Chinese journal of Epidemiology,2010,31(1):47-50

北京市郊区八种动物戊型肝炎病毒抗体流行率调查及病毒基因型研究

Hepatitis E virus(HEV)genotype and the prevalence of anti.HEV in 8 species of animals in the suburbs of Beijing

Received:September 09, 2009

DOI：

KeyWord: 戊型肝炎病毒抗一HEV 基因型人兽共患病

English Key Word: Hepatitis E virus Anti-HEV antibody Genotype Zoonosis

FundProject:国家高技术研究发展计划(863计划)(2006AA02ZA53);国家自然科学基金(30570063)

Author Name	Affiliation	E-mail
GENG Jiabao	Peking University Health Science Center. Beijing 100191. China
FU Hongwei	Peking University Health Science Center. Beijing 100191. China
WANG Ling	Peking University Health Science Center. Beijing 100191. China	lingwang@bjmu.edu.cn
WANG Xiaojuan	Peking University Health Science Center. Beijing 100191. China
GUAN Jianmin	Beijing Huairou Districtal Center for Animal Disease Control
CHANG Yibin	Peking University Health Science Center. Beijing 100191. China
LI Lingjun	Peking University Health Science Center. Beijing 100191. China
ZHU Yonghong	Peking University Health Science Center. Beijing 100191. China
ZHUANG Hui	Peking University Health Science Center. Beijing 100191. China	zhuangbmu@126.com
LIU Quanhong	Beijing Huairou Districtal Center for Animal Disease Control
PENG Xingchun	Beijing Huairou Districtal Center for Animal Disease Control

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Abstract:

目的调查北京地区与人密切接触的猪、牛、羊、马、驴、犬、鸡、鸭8种动物血清中戊型肝炎病毒(HEY)抗体流行率及该地区猪HEV基因型和亚型。方法收集8种动物的血清标本及幼猪粪便标本，用双抗原夹心ELISA法检测血清中抗一HEV；用巢式反转录聚合酶链反应法(RT-nPCR)检测HEV RNA；对部分PCR产物进行克隆和测序，并对测序结果进行基因分型。结果在8种动物中抗一HEV阳性率分别为猪80.43％(481/598)，其中成猪(>6月龄)为87.86％(369/420)，幼猪(2.3月龄)为62.92％(112/178)；牛15.02％(52/346)；马14.29％(40/280)；驴0(0/26)；羊9.88％(33/334)；犬0(0/21)；鸭3.03％(7/231)；鸡2.53％(8/316)。RT-nPCR检测3月龄以下猪粪便标本HEVRNA阳性率为66.67％(74/111)。HEVRNA阳性的标本测序后可归为6株(分别命名为蛳伽1、蛳w2、bjsw3、够w4、蜘w5和bjsw6)，6株HEV开放读码框2(ORF2)部分核苷酸序列的相似性为94.5％～99.6％，与1、2、3、4型HEV的相似性分别为75.6％一78.6％、75.6％～76.2％、77.1％一80.7％和83.7％一94.5％。6株HEV与人HEV4d亚型的同源性最高，为90.O％～94.5％。结论北京市郊区猪、牛、马、羊、鸭、鸡中均存在HEV感染，其中猪抗一HEV流行率最高，驴、犬抗一HEV的流行率最低；6株猪HEV属于基因4型、4d亚型。

English Abstract:

Objecfive To investigate the prevalence of anti—hepatitis E virus(HEV)and genotypes of hepatitis E virus in 8 species ofanimals including swine，cattle，sheep，horse，donkey，dog，chicken and duck in the suburb of Beiiing.Methods Serum samples were collected from the 8 species of animals.and fecal samples of younger swine were collected from 2 stock farms.Anti-HEV was detected by Double Antigen Sandwich Assay.HEV RNA from fecal samples was detected by a reverse transcription nested polymerase chain reaction(Rr-nPCR).Parts of the PCR products were cloned and sequenced.111e swine HEV seqHeliCeS were analyzed genetically.Results 111e positive rates of anti—HEV in sel'Ulll specimens of swine，cattle，horse，donkey，sheep，dog，duck and chicken were 80.43％(48l/598)，15.02％(52/346)，14.29％(401280)，0(0/26).9.88％(33/334)，0(0/21).3.03％(7/231)and 2.53％(8/316)，respectively.The anti—HEV prevalence of adult swine(≥6months)and younger swine(≤3 months)were 87.86％(369/420)and 62.92％(112/178)respectively.74 of ll1(66.67％)pig faces were positive for HEV RNA.Sequence analysis on these positive samples showed that there were 6 groups of HEV designated as bjswl，bjsw2，bjsw3，bjsw4，bjsw5 and bjsw6.Thee 6 strains of HEV shared 94.5％-99.6％sequence identity of partial HEV ORF2 nucleotide with each other.The identities of HEV ORF2 nueleotide sequences between the 6 strains and genotye l，2，3 and 4 were 75.6％一78.6％.75.6％一76.2％，77.1％一80.7％and 83.7％一94.5％，respectively.The sequence identity between the 6 strains and human HEV genotype 4d was 90.0％一94.5％.Conclusion HEV infection Was Seen in swine，cattle，horse，sheep.duck and chicken in the suburbs of Beijing.nle anti·HEV positive rate appeared the highest in swine and the lowest in dog and donkey.The six strains of HEV isolated from younger swine belonged to genotype 4d.

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