| 王晓光,雷永良,陈秀英,孟胜利,唐青.狂犬病病毒浙江街毒株糖蛋白基因序列分析[J].Chinese journal of Epidemiology,2010,31(1):73-78 |
| 狂犬病病毒浙江街毒株糖蛋白基因序列分析 |
| Sequencing and analyses on glycoprotein gene of rabies viruses isolated in Zhejiang province，China |
| Received:August 14, 2009 |
| DOI： |
| KeyWord: 狂犬病病毒 糖蛋白 基因序列 |
| English Key Word: abies virus Glycoprotein Gene sequencing |
| FundProject:浙江省科技厅2009年度一般科研计划项目(2009C33166)；公益性行业(农业)科研专项(200803014)；国家高技术研究发展计划(863计划)(sQ2006AA022112882)；公共卫生应急检测关键技术重点实验室开放课题 |
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| Abstract: |
| 目的测定狂犬病病毒浙江株(鼬獾源和犬源)糖蛋白(GP)基因组全序列，从分子水平比较狂犬病病毒浙江株与其他地区代表性街毒株和疫苗株GP之间的差异。方法乳鼠接种分离狂犬病病毒，RT-PCR反应测定狂犬病病毒浙江株GP基因核苷酸序列，并进行序列和编码蛋白的比较分析。结果测序获得浙江5株鼬獾源狂犬病病毒和9株犬源狂犬病病毒GP基因序列，全长1575个核苷酸，编码524个氨基酸。浙江病毒株与其他地区街毒株、疫苗株核苷酸和氨基酸序列相似性在82.3％～99.9％和85.1％一99.8％之间，种系分析显示浙汀株均为基因l型，GP编码蛋白无重组，主要抗原位点未出现较大的变异。结论对GP基因一级结构综合分析表明，在某些区域存在优势抗原表位的可能性较大，可能出现潜在的蛋白质抗原决定簇，浙江株GP基因变异较小，与国内其他地区流行的代表性街毒株相似，但高于其他疫苗株，在基因结构及种系进化关系上存在一定的距离。 |
| English Abstract: |
| 0bjective Based on sequencing the genomes of glycoprotein(GP)gene ofrabies viruses isolated in Zhejiang.we analyzed the properties of rabies viruses genetic variation in molecular level，and to compare with those of other representative vaccine strains and street virus strains.get the information about rabies viruses variation.Methods Suckling mice against rabies virus were selected.Overlapped fragments were amplified bv RT-PCR and full-1ength genomes were assembled to analyze the nucleotide and deduced protein similarities and phylogenetic analyses of the GP genes.Results The fourteen full.1ength genomes were completely sequenced and they had the sanle genetic structure with 1 575 nts and deduced protein with 524 aa.Genetic analysis revealed that the nucleotide and amino acid homologies of GP gene from Zhejiang strains and other vaccine strains or street virus strains were 82.3％一99.9％and 85.1％一99.8％.The fourteen strains were genotype l according to the phylogenetic analyses.The GP amino acids of Zhejiang sWains rabies virus strains without any recombination occurred in GP and no larger variation appeared in the major antigenic sites.Conclusion The comprehensive analysis based on the first—level structure of GP demonstrated that it was possible that some advantageous antigenic epitopes existed in certain areas and potential antigenic determinants.It was evident that the GP gene of Zhejiang strains appear to be stable and meir sequence similarity with the representative strains of street virus in China were higher than those of other vaccine strains.Some differences showed in the genetic structure and evolution relationship among Zhejiang strains，other street strains in other regions and vaccine strains. |
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