白介素-1O、干扰素-γ基因多态性与肺结核病易感性的关系

杨慧; 梁肇海; 刘小立; 王峰

Abstract

杨慧,梁肇海,刘小立,王峰.白介素-1O、干扰素-γ基因多态性与肺结核病易感性的关系[J].Chinese journal of Epidemiology,2010,31(2):155-158

白介素-1O、干扰素-γ基因多态性与肺结核病易感性的关系

Association between polymorphisms of interleukin一10，interferon一^r gene and the susceptibility to pulmonary tuberculosis

Received:August 14, 2009

DOI：

KeyWord: 肺结核病白细胞介素一10 干扰素-γ 基冈多态性

English Key Word: Pulmonary tuberculosis Interleukin-10 Interferon-γ, Gene polymorphism

FundProject:

Author Name	Affiliation	E-mail
YANG Hui	Shenzhen Center for Chronic Disease Control and Prevemion, Shenzhen 518020, China	yh2009en@yahoo.com.cn
LIANG ZhaoHai	Futian Center for Disease Control and Prevention
LIUXiao-li	Shenzhen Center for Chronic Disease Control and Prevemion, Shenzhen 518020, China
WANG Feng	Shenzhen Center for Chronic Disease Control and Prevemion, Shenzhen 518020, China

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Abstract:

目的探讨白介素一10(IL一10)基因一1082G/A位点和干扰素-γ(IFN吖)基因+874A/T位点单核苷酸多态性(SNP)与肺结核病易感性的关系。方法采用序列特异性引物PCR(PCR.SSP)及测序技术检测深圳地区汉族人群肺结核病例组及对照组各200例IL-IO启动子区一1082G/A、IFN-γ+874A，r位点基因多态性。结果病例组IL-i0(一1082)位点A/A纯合子、刖G杂合子、G/G纯合子基因型频率分别为85.4％、13.1％、1.5％，对照组分别为77.5％、22.O％、0.5％；病例组A、G等位基冈频率分别为91.9％、8.1％，对照组分别为88.5％、11.5％。两组问基因型分布差异有统计学意义(P<0.05)。病例组A/A基因型高于对照组，G/A基因型低于对照组(P<0.05)。但两组的A、G等位基因频率分布差异没有统计学意义。IFN-γ(+874)位点基因型频率及等位基因频率在病例组和对照组中分布无明显差异(P>0.05)。结论中国汉族人群中细胞冈子IL一10(一1082)基因SNP与肺结核易感性相关。A/A基因型可能是一个肺结核易感的风险因素。细胞因子IFN-γ(+874)基因位点SNP与肺结核易感性无关。

English Abstract:

0bjective To investigate the association of IL-10，IFN-γ gene polymorphisms at position-1082G/A andA/T were and susceptibility to pulmonary tuberculosis(PTB)through a case-control study.Methods Polymerase chain reaction with sequence specific primer(PCR.SSP)/sequencing method，single nucleotide polymorphisms(SNP)of IL一10 and IFN-γgene atposition一1082 and+874 were used on pulmonary tuberculosis patients(group PTB)and normal healthy subjects(group NHS)in the Han population from Shenzhen eity of China.Results The genotype frequencies of A/A homozygotes.A/G heterozygous and G/G homozygous at-1082 site of IL一10 gene in patients with pulmonary tuberculosis were 85.4％.13.1％.1.5％respectively and A，G allele frequencies were 91.9％.8.1％respectively.In healthy subjects，the genotype frequencies ofA/A homozygotes，A/G heterozygous and G/G homozygous were 77.5％，22.0％，O.5％respectively and A。G allele frequencies were 88.5％，11.5％respectively.Genotype distribution between the two groups was significantly different(P0.05).For IFN-γ(+874)and no significant difference Was found on the distribution ofgenotypes or alleles between the two groups.Conclusion The IL-10(一1082) gene SNP might be associated with the susceptibility to tuberculosis.AYA genotype might be a risk factor for the susceptibility on tuberculosis while the SNP ofeytokines(+874)might not be associated with the susceptibility to tuberculosis in Chinese Han populations.

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