浙江省1 998年H3N2流感流行的溯源研究

冯燕; 徐昌平; 黄崴; 钟淑玲; 严菊英; 莫世华; 卢亦愚

Abstract

冯燕,徐昌平,黄崴,钟淑玲,严菊英,莫世华,卢亦愚.浙江省1 998年H3N2流感流行的溯源研究[J].Chinese journal of Epidemiology,2010,31(2):204-207

浙江省1 998年H3N2流感流行的溯源研究

Traceability of the xnj3N2 virus borne influenza pandemic in Zhejiang province，1998

Received:August 17, 2009

DOI：

KeyWord: 流感病毒遗传距离抗原比溯源分析

English Key Word: Influenza virus A/H3N2 Genetic distance Antigenic ratio Traceability

FundProject:浙江省自然科学基金重点项日(Z303909)

Author Name	Affiliation	E-mail
FENG Yan	Virus Institute of Zhejiang ProvinciaZ Centre for Disease Control and Prevention, Hangzhou 31005l, China
XU Chang-ping	Virus Institute of Zhejiang ProvinciaZ Centre for Disease Control and Prevention, Hangzhou 31005l, China
HUANG Wei	Department ofLab Medicine of Wenzhou Medical College
ZHONG Shu-ling	Virus Institute of Zhejiang ProvinciaZ Centre for Disease Control and Prevention, Hangzhou 31005l, China
YAN Ju-ying	Virus Institute of Zhejiang ProvinciaZ Centre for Disease Control and Prevention, Hangzhou 31005l, China
LU Yi-yu	Virus Institute of Zhejiang ProvinciaZ Centre for Disease Control and Prevention, Hangzhou 31005l, China
卢亦愚	Virus Institute of Zhejiang ProvinciaZ Centre for Disease Control and Prevention, Hangzhou 31005l, China	luyiyuzjh@yahoo.com.cn

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Abstract:

目的对1998年浙江省的H3N2流感流行进行溯源研究。方法采用RT-PcR扩增浙江省1998年3株H3N2流感流行代表株的全基因组序列，并与Gerd3ank上1995-1998年世界其他地区H3N2流感流行株进行比较；同时，采用交叉血凝抑制实验，计算各毒株问的抗原比。结果HA基因进化树表明，1998年浙江省H3N2优势流行株A/Zhejiang/11/98、A/Zhejiang/18/98与1995-1996年世界各地以及1997年我国大陆的H3N2流行株间存在显著差异，在进化树上虽与A/Sydney/5/97同属一簇，但和美国纽约以及中国香港1997年后期流行株更为接近。在HAl、NA和MP基凶上，A/Zhejiang/18/98与香港1997年后期流行株同源性最高，而在PA、HA和NS基因上，与纽约流行株的遗传距离也小于A/Sydney/5/97。A/Zhejiang/I 8/98与香港或纽约株在HAl区仅存在l～3个位点的氨基酸残基不同，而与A/Sydney/5/97存在7个位点的氨基酸残基差异，其中3个位点于抗原决定簇区。各毒株问的交叉II}L凝抑制实验表明A/Zhejiang/18/98与A/Sydney/5/97的抗原比已达2.0，提示二者在抗原性上存在一定差异。此外，1997-1998年H3N2各地流感流行的起始时间序列，也显示了该次流感传播的可能途径。结论浙江省1998年H3N2流感的流行很可能是由1997年底H3N2新型流感变异株经纽约和香港输入中国大陆所导致。

English Abstract:

0bjective To trace back to the influenza pandemic caused by A/H3N2 virus happened in Zhejiang province.1 998.Methods The whole genome of three isolates related to Zheiiang influenza virus was amplified through RT-PCR,and the identified sequences were aligned with the sequences downloaded from GenBank of the H3N2 strains which were circulating in other regions during l 995 to l 998.The crossing HAI titers of the reference strains were measured by HAI test and antigenic ratios were calculated.Results The Phylogenetic tree.constructed based Oll HA sequence showed that the dominant strains A/zhejianF/ll/98 and A/Zhejiang/l 8/98 were significant different from the isolates circulated in other regions dudng 1995 to 1996 and the strains isolated in the mainland of China.in 1997.Although the A/Zhejiang/l 1/98 and A/Zhejiang/l 8/98 strains were distributed in the same cluster with A/Sydney/5/97.the two strains were closer to the epidemic strains isolated in Hong Kong and New York in the later part of 1997.Based on HAl，NA and MP genes，～Zhejiang/18/98 seemed to be the closest one to the Hong Kong epidemic strains，and the genetic distances between A/Zhejiang/l 8/98 and New York strains were shorter than that with A/Sydney/5/97 based on PA.HA and NS genes.There were only 1-3 amino acid differences between A/Zhejiang/1 8/98 and Hong Kong or New York strains.whereas 7 amino acid differences with A/Sydney/5/97，in which three were located in the antigenic detcrminant regions.Data from the crossing HALl test showed that the antigenic ratio between A/Zheiiang/l 8/98 and A/Sydney/5/97 had reached 2.0.indicating the antigenic difference to a certain extent.Additionally。the onset of the influenza epidemic during 1 997 to l 998 also suggested the possible route of transmission related to this H3N2 virus.Conclusion The influenza pandemic occurred in Zhejiang province in 1 998 was possibly caused by the importation of a newly identified H3N2 influenza variant via Hong Kong and New York in late 1997.

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