| 靳淼,孙军玲,常昭瑞,李慧莹,刘娜,章青,崔淑娴,张静,王子军,段招军.中国2006—2007年诺如病毒胃肠炎暴发及其病原学特征分析[J].Chinese journal of Epidemiology,2010,31(5):549-553 |
| 中国2006—2007年诺如病毒胃肠炎暴发及其病原学特征分析 |
| Outbreaks of noroviral gastroenteritis and their molecular characteristics in China, 2006一2007 |
| Received:November 18, 2009 |
| DOI：10.3760/cma.j.issn.0254-6450.2010.05.017 |
| KeyWord: 诺如病毒 胃肠炎 暴发 基因分型 |
| English Key Word: Norovirus Gastroenteritis Outbreak Genotype |
| FundProject:国家科技重大专项(2008ZXl0004—013) |
| Author Name | Affiliation | E-mail | | JIN Miao | lnstituxe for Yiral Diseases Control Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052 | | | SUN Jun-ling | Chinese Center Disease Control and Prevention | | | CHANG Zhao-rui | Chinese Center Disease Control and Prevention | | | LI Hui-ying | lnstituxe for Yiral Diseases Control Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052 | | | LIU Na | lnstituxe for Yiral Diseases Control Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052 | | | ZHANG Qing | lnstituxe for Yiral Diseases Control Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052 | | | Shu-xian | lnstituxe for Yiral Diseases Control Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052 | | | ZHANG Jing | Chinese Center Disease Control and Prevention | | | WANG Zi-jun | Chinese Center Disease Control and Prevention | | | DUAN Zhao-jun | lnstituxe for Yiral Diseases Control Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052 | zhaojund@126.Com |
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| Abstract: |
| 目的了解中国2006--2007年诺如病毒胃肠炎暴发及其病毒基因型别。方法 收集2006—2007年19起胃肠炎暴发的流行病学资料以及腹泻粪便样本，用RT-PCR方法对201份粪便标本进行诺如病毒、轮状病毒、星状病毒、腺病毒和札如病毒检测，对扩增产物进行序列测定。用Clustal X 1.83和MEGA4.0生物软件对诺如病毒序列进行序列比对和系统进化分析。结果 诺如病毒是引起病毒性胃肠炎暴发的主要病原之一(12/19，63.2%)。在12起诺如病毒胃肠炎暴发中，变异株GⅡ-4/2006b是引起暴发的流行优势株(11/12，91.7%)，其他型别包括GⅡ-17、GⅡ-6和GⅡ-3。诺如病毒引起的疫情暴发主要集中在冬春季节(12月至4月)，发生场所多样，且各年龄组人群均有发病。同时，12起诺如病毒暴发中有2起与其他腹泻病毒混合感染，且在同一起暴发中病原具有病毒多样性和基因型别多样性。结论 诺如病毒是2006—2007年引起胃肠炎暴发疫情的主要病原之一，变异株GⅡ-4/2006b为流行优势株。 |
| English Abstract: |
| Objective To acknowledge the epidemiology of gastroenteritis outbreaks caused by norobiruses and their genotypes.Methods Epidemiologic data and specimens were collected from 19 gastroenteritis outbreaks.201 specimens were detected for norovirus,rotavirus,astrovirus,adenovirus and sapovirus妙RT PCR methods and PCR products were sequenced.Sequence alignment and phylogenetic analysis were performed by Clustal X 1.83 and MEGA 4.0 programs.Results Noroviruses were one of the most predominant pathogens causing viral gastroenteritis outbreaks(12 of 19 outbreaks,accounting for 63.2%)Variant GⅡ-4/20066 was the predominant strain responsible for 11 of the 12 NV-associated outbreaks.Other genotypes would include GⅡ-17,GⅡ-6 and GⅡ-3.The NV-associated gastroenteritis outbreaks occurred mainly in winter and spring between December 2006 and April 2007.These gastroenteritis outbreaks caused by noroviruses would involve all age groups in various locations.Meantime,2 out of 12 outbreaks were caused by norovirus or other viruses.In addition,multiple viruses and multiple genotypes of notoviruses were found in the same outbreak.Conclusion Noroviruses were one of the most major pathogens causing gastroenteritis outbreaks while GⅡ-4/2006b variant was identified as the predominant strain in China. |
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