| 夏玉刚,陆一涵,胡安群,秦雪,董晓莲,朱建福,姜庆五,郑英杰.华东和华南地区商品猪戊型肝炎病毒携带率和基因分型分析[J].Chinese journal of Epidemiology,2010,31(7):791-794 |
| 华东和华南地区商品猪戊型肝炎病毒携带率和基因分型分析 |
| Study on the prevalence and genotype of hepatitis E virus among commercial swine population in Eastern and Southern China |
| Received:January 11, 2010 |
| DOI： |
| KeyWord: 戊型肝炎病毒 携带率 基因型 猪 |
| English Key Word: Hepatitis E virus Prevalence Genotype Swine |
| FundProject:国家自然科学基金(30771842，30872158)；上海市重点学科项目(Bll8 |
| Author Name | Affiliation | E-mail | | XIA Yu-gang | Department of Epidemiology, School of Public Health, Fudan University, the Key Laboratory of Public Health Safety, Ministry of Education, Shanghai 200032, China | | | LU Yi-han | Department of Epidemiology, School of Public Health, Fudan University, the Key Laboratory of Public Health Safety, Ministry of Education, Shanghai 200032, China | | | HU An-qun | Department of Epidemiology, School of Public Health, Fudan University, the Key Laboratory of Public Health Safety, Ministry of Education, Shanghai 200032, China | | | QIN Xue | Department of Epidemiology, School of Public Health, Fudan University, the Key Laboratory of Public Health Safety, Ministry of Education, Shanghai 200032, China | | | DONG Xiao-lian | Anqing Municipal Hospital of Anhui Province | | | ZHU Jian-fu | The First Affliated Hospital of Guangxi Medical University | | | JIANG Qing-wu | Center for Disease Control and Prevention of Deqing County, Zhejiang Province | | | ZHENG Ying-jie | Center for Disease Control and Prevention of Deqing County, Zhejiang Province | yjzheng@shmu.edu.cn |
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| Abstract: |
| 目的 了解华东和华南地区商品猪戊型肝炎病毒(HEV)的携带率和基因分型.方法 2007-2009年收集华东和华南地区5家屠宰场商品猪胆汁600份,应用巢式RT-PCR方法检测HEVRNA,测序后做系统进化分析.结果 600份猪胆汁中有47份(7.83%)分离出HEVRNA.基于HEV ORF2区域内150-nt序列的系统进化分析结果,分离的47份HEV病毒株均属于Ⅳ型,与Ⅰ、Ⅱ、Ⅲ和Ⅳ型参考病毒株的核苷酸同源性分别为75.0%～83.4%、75.0%～84.6%、71.9%～80.7%和88.1%～91.5%,且大部分病毒株按采样地区来源表现出地区聚集性.结论 HEV在华东和华南地区的商品猪中广泛存在,应加强对商品猪食用安全问题的关注. |
| English Abstract: |
| Objective To determine the prevalence and genotype of hepatitis E virus (HEV)among commercial swine population in Eastern and Southern China.Methods Six hundred specimens of swine bile collected from 5 slaughterhouses in Eastern and Southern China from 2007 to 2009 were tested for HEV RNA using nested RT-PCR. PCR products were sequenced for phylogenetic analysis. Results Forty-seven out of the 600 samples (7.83%) were positive for HEV RNA. Based on the 150 nt fragment within HEV ORF2, data from phylogenetic analysis revealed that all the 47 HEV isolates were identified to be genotype Ⅳ, sharing 75.0%-83.4%, 75.0%-84.6%, 71.9%-80.7% and 88.1%-91.5% nucleotide identities with prototype Ⅰ,Ⅱ,Ⅲ and Ⅳ HEV strains respectively while majority of the isolates clustered within their respective isolation sites. Conclusion HEV was widespread in commercial swine population in Eastern and Southern China that raised a serious concern about the safety regarding the consumption of pork products. |
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