| 耿震,侯学霞,万康林,郝琴.中国六省蜱中莱姆病螺旋体分离与鉴定[J].Chinese journal of Epidemiology,2010,31(12):1346-1348 |
| 中国六省蜱中莱姆病螺旋体分离与鉴定 |
| Isolation and identiffcation of Borrelia burgdorferi seilsu/ato from ticks in six provinces in China |
| Received:March 24, 2010 |
| DOI：10.3760/ema.j.issn.0254-6450.2010.12.005 |
| KeyWord: 莱姆病 螺旋 蜱 |
| English Key Word: Lyme disease Borrelia burgdorferi sensu Tick |
| FundProject:“十一五”国家科技支撑计划(2008ZXl0004-010) |
| Author Name | Affiliation | E-mail | | Gen Zhen | National Institute for Communicable Disease Control and Prevention,chinese Center for Disease Control and Prevention,Beijing 102206,China | | | Hou Xuexia | National Institute for Communicable Disease Control and Prevention,chinese Center for Disease Control and Prevention,Beijing 102206,China | | | Wan Kanglin | National Institute for Communicable Disease Control and Prevention,chinese Center for Disease Control and Prevention,Beijing 102206,China | | | Hao Qin | National Institute for Communicable Disease Control and Prevention,chinese Center for Disease Control and Prevention,Beijing 102206,China | haoqin@icdc.cn |
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| Abstract: |
| 目的了解中国6省蜱中莱姆病螺旋体携带情况.方法在6省各选取2个采样点捕蜱,采用病原分离培养和巢式PCR方法对蜱进行莱姆病螺旋体检测,通过基因测序确定分型.结果6省共采集到2200余只蜱,约1000只蜱用于病原分离培养,从吉林省长白县全沟硬蜱标本中分离到13株螺旋体.从贵州省道真粒形硬蜱标本中分离到9株螺旋体.1255只蜱用于PCR检测,从6省的蜱标本中检测到莱姆病螺旋体特异片段,其中吉林省(长白县27.08%、通化县.41%)、青海省(互助县25.06%、黄南县21.1l%)和贵州省(道真县25.63%)蜱中莱姆病螺旋体阳性率较高,山西省(垣曲县4.72%、交城县3."%)蜱中莱姆病螺旋体阳性率较低.通过序列同源性分析确定吉林、青海、甘肃和山西省蜱中莱姆病螺旋体基因型均为Borreliagarinii.贵州、湖南省的基因型均为Borrelia valaisiana.结论6省蜱中均带有莱姆病螺旋体,且带菌率有差异;山西省蜱中存在Borreliagarinii型莱姆病螺旋体,湖南省蜱中存在Borreliavalaisiana型莱姆病螺旋体. |
| English Abstract: |
| Objective To understand the carrying status of Borrelia burgdorferi in ticks from the mountain areas from six representative provinces，including Jilin，Shanxi，Gansu，Qinghai， Guizhou and Hunan in China．Methods Flagging and trapping methotis were used to collect ticks in forest area and culture medilllll was used to isolate the pathogen．Nested-PCR Was used to detect the germ-carrying rate of tieks．Resuits More tllan 2200 ticks from six representative provinces were collected and 1000 tieks were used to isolate the pathogen．13 Lyme disease spirochetes from ixodes persuicatus in Changbai．Jilin province and 9 Lyme disease spirochetes from ixodes granulatus in Daozhen．Guizhou province were identified．There were 1255 ticks used for PCR testing．Specific fragments of the Borrelia burgdooCeri in ticks were found from the six representative provinces in China．111e cartier rate Was higher in Jilin(Changbai 27．08％。TotIgbua 20．41％)，Qinghai(Huzhu 25．06％。Huangnan 21．1l％)and Guizhou(Daozhen 25．63％)，than in Shanxi(Yuanqu 4．72％， Jiaocheng 3．64％)．Result from the sequence analysis showed that the genotype belong to Borrelia garinii in Jilin．Qinghai，Gansu，Shanxi provinces but Borrelm valaisiana in Guizhou and Hunan provinces．Condusion Our data showed that there existed Lyme disease spirochetes in a11 the six representative provinces in China，but the carriying rates of ticks were different．Borrelia garinii Was found in Shanxi province。and Borrelm valaisiana in Hunan province． |
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