埃可病毒11型山东株分子流行病学研究

樊庆莹; 王海岩; 杨赫; 冯艺; 张岩; 李岩; 陶泽新; 纪峰; 宋立志; 刘桂芳; 刘尧; 冯蕾; 徐爱强

Abstract

樊庆莹,王海岩,杨赫,冯艺,张岩,李岩,陶泽新,纪峰,宋立志,刘桂芳,刘尧,冯蕾,徐爱强.埃可病毒11型山东株分子流行病学研究[J].Chinese journal of Epidemiology,2011,32(2):155-158

埃可病毒11型山东株分子流行病学研究

Study on the genetic characterization and molecular epidemiology of echovirus 11 strains isolated in Shandong province,China

Received:October 13, 2010

DOI：

KeyWord: 埃可病毒11型基因特征分子流行病学

English Key Word: EChovirus ll Genetic characterization Molecular epidemiology

FundProject:国家科技重大专项(2009ZXl0004-202)；山东省医药卫牛重点项目计划课题(2007HZ090)

Author Name	Affiliation	E-mail
FAN Qing-ying	Institute of Epidemiology and Health Statistics, School of Public Health Shandong University, Jinan 250012. China
WANG Hai-yan	Shandong Provincial Center for Disease Control and Prevention
YANG He	Institute of Epidemiology and Health Statistics, School of Public Health Shandong University, Jinan 250012. China
FENG Yi	Shandong Provincial Center for Disease Control and Prevention
ZHANG Yan	Shandong Provincial Center for Disease Control and Prevention
LI Yan	Shandong Provincial Center for Disease Control and Prevention
TAO Ze-xin	Shandong Provincial Center for Disease Control and Prevention
JI Feng	Shandong Provincial Center for Disease Control and Prevention
SONG Li-zhi	Shandong Provincial Center for Disease Control and Prevention
LIU Gui-fang	Shandong Provincial Center for Disease Control and Prevention
LIU Yao	Shandong Provincial Center for Disease Control and Prevention
FENG Lei	Shandong Provincial Center for Disease Control and Prevention
XU Ai-qiang	Shandong Provinciat Key Laboratory of Infectious Disease Control and Prevention	aqxuepi@163．Com

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Abstract:

目的分析埃可病毒11型(Echo11)山东分离株的分子流行病学特征.方法对1994-2009年山东省急性弛缓性麻痹监测系统中采集到的粪便标本,采用RD、Hep-2和L20B 3种细胞进行病毒分离;对阳性分离物采用中和试验确定其血清型别;选取27株Echo11山东株病毒,测定VP1完整编码区序列,与国外其他病毒株进行同源性分析并构建VP1区全基因亲缘进化树,分析其进化来源及不同基因型的流行范围.结果 1994-2009年山东省急性弛缓性麻痹病例标本共分离出 915株已确定血清型的非脊髓灰质炎肠道病毒(NPEV),其中Echo11 79株,占8.6%.Echo11山东株VP1区全长共876个核苷酸,编码292个氨基酸;27株Echo11 VP1区山东株之间核苷酸同源性为76.4%～100.0%,氨基酸同源性为91.4%～100.0%;与原型Gregory株之间核苷酸同源性为77.7%～80.7%,氨基酸同源性为90.7%～94.8%.结论山东省存在Echo11 A基因型和C基因型流行,并构成A1、A2和C1三个新基因亚型,且不同亚型内存在着多条传播链,A1亚型为近年山东省流行的基因亚型.

English Abstract:

Objective To analyze the evolution and genetic characterization of echovirus 11 (Echo11 ) from the acute flaccid paralysis (AFP) cases in Shandong province. Methods Isolation of Enterovirus was performed from stool samples of AFP cases from 1994 to 2009. All positive strains were sero-typed by neutralization test. Entire VP1 coding region from 27 strains typed as Echo 11 was amplified by reverse transcription-polymerase chain reaction(RT-PCR), and subsequently phylogenetic analyse on VP1 sequences from these strains and others published in GenBank were conducted. Results From 1994 to 2009, altogether 915 non-polio enterovirus (NPEV) strains were isolated with 79(8.6% ) isolates serotyped as Echo11. There were 876 nucleotides (nt) in the complete VP1 genes, encoding 292 amino acids (aa). The nt identities were 76.4%-100.0% among those Echo11 Shandong strains with the aa identities as 91.4% -100.0%. The nt and aa identities were 77.7%-80.7% and 90.7%-94.8% between Shandong strains and prototype strains, respectively.Conclusion All Echo11 strains could be divided into four genotypes. Shandong strains that forming three (A1, A2 and C1) new sub-genotypes, with every sub-genotype had several brands.Sub-genotype A1 appeared to be the lately circulating one.

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