| 刘芮汐,李奇志,幸琳琳,彭哲,朱朝敏.MIRU-VNTR和Spoligotyping用于重庆地区210株儿童结核分枝杆菌临床分离株的基因分型[J].Chinese journal of Epidemiology,2011,32(6):593-597 |
| MIRU-VNTR和Spoligotyping用于重庆地区210株儿童结核分枝杆菌临床分离株的基因分型 |
| Genotyping of 210 Mycobacterium tuberculosis strains with Spoligotyping and MIRU-VNTR among pediatric tuberculosis patients in Chongqing |
| Received:December 18, 2010 |
| DOI： |
| KeyWord: 结核分枝杆菌 北京基因型 数目可变串联重复序列 间隔区寡核苷酸分型 儿童 |
| English Key Word: Mycobacterium tuberculosis Beijing genotype Mycobaeterial interspersed repetitive unit-variable-number tandem repeat Spoligotyping Children |
| FundProject:重庆市卫生局科研基金(2008-2-177) |
| Author Name | Affiliation | E-mail | | LIU Rui-xi | Division of Infectious Disease and Gastroenterology, Children' s Hospital, Chongqing Medical University, Key Laboratory of Developmental Disease in Childhood Ministry of Education, Key-Laboratory of Pediatrics in Chongqing, CSTC2009CA5002
Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China | | | LI Qi-zh | Division of Infectious Disease and Gastroenterology, Children' s Hospital, Chongqing Medical University, Key Laboratory of Developmental Disease in Childhood Ministry of Education, Key-Laboratory of Pediatrics in Chongqing, CSTC2009CA5002
Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China | | | XING Lin-lin | Division of Infectious Disease and Gastroenterology, Children' s Hospital, Chongqing Medical University, Key Laboratory of Developmental Disease in Childhood Ministry of Education, Key-Laboratory of Pediatrics in Chongqing, CSTC2009CA5002
Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China | | | PENG Zhe | Division of Infectious Disease and Gastroenterology, Children' s Hospital, Chongqing Medical University, Key Laboratory of Developmental Disease in Childhood Ministry of Education, Key-Laboratory of Pediatrics in Chongqing, CSTC2009CA5002
Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China | | | ZHU Chao-min | Division of Infectious Disease and Gastroenterology, Children' s Hospital, Chongqing Medical University, Key Laboratory of Developmental Disease in Childhood Ministry of Education, Key-Laboratory of Pediatrics in Chongqing, CSTC2009CA5002
Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China | zhuchaomin@yahoo.com.cn |
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| Abstract: |
| 目的 评价间隔区寡核苷酸分型(Spoligotyping)和基于结核分枝杆菌散在分布数目可变串联重复序列分析(MIRU-VNTR)方法在重庆地区儿童结核病分子流行病学中的应用.方法 收集重庆地区210株儿童结核分枝杆菌(MTB)临床分离株,应用上述两种分型方法进行比较分析.结果 采用Spoligotyping分型方法,210株菌可分为2个基因群44种基因型,其中最大的1个基因群即北京家族(北京基因型)含有130株菌(61.90%).采用MIRU-VNTR分析发现24个位点的多态性差异较大,不同MIRU位点组合(12、15和24位点)的分辨率指数依次升高,后两个组合的差异是由位点ETR-B引起.各位点和各位点组合在北京家族菌株中的分辨率指数均高于非北京家族菌株.结论 重庆地区儿童MTB具有明显的基因多态性,其主要流行型为北京家族.在结核病原学监测中,可先采用Spoligotyping,再对成簇菌株进行15位点与ETR-B组合二次分型的联合分型策略,可提高分子流行病学调查效果. |
| English Abstract: |
| Objective To evaluate the application of spacer oligonucleotide typing (Spoligotyping) and mycobaeterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) analysis in the molecular-epidemiological study of tuberculosis and to discuss the characteristics of pediatric Mycobacterium (M.) tuberculosis strains in Chongqing.Methods M. tuberculosis strains isolated and typed by Spoligotyping and MIRU-VNTR respectively, from the children patients in Chongqing and to compare the results from both methods, epidemiologically. Results By means of Spoligotyping, 210 clinical isolates were divided into 2 gene groups, displaying 44 genotypes. Among them, the biggest group was M. tuberculosis Beijing family, including 130 strains (61.90%),using the Spoligotyping. From the results of MIRU-VNTR, 24 loci showed different polymorphism and the HGI of different loci set (12 old loci, 15 basic loci and 24-loci set) increased accordingly. The subtle difference in HGI was originated from one locus ETR-B, which was included in the 24-locus system. The diversity of each loci and MIRU-VNTR set for non-Beijing genotype strains was higher than that of the Beijing genotype strains. Conclusion In this study, it was preliminarily confirmed the existence of high polymorphism of M. tuberculosis while the Beijing Family was the main genotype and main prevalent strain in children of Chongqing area. Spoligotyping prior to 15-locus with ETR-B combination seemed more suitable for the massive epidemiological investigation of pediatric tuberculosis patients. |
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