| 王淼若,王文,林献丹,梅盛华,郭文平,张永振.浙江省龙泉市汉坦病毒宿主动物自然感染状况研究[J].Chinese journal of Epidemiology,2011,32(6):598-601 |
| 浙江省龙泉市汉坦病毒宿主动物自然感染状况研究 |
| Investigation on the natural infectious status of hantaviruses among small mammals in Longquan city, Zhejiang province |
| Received:January 20, 2011 |
| DOI： |
| KeyWord: 肾综合征出血热 汉坦病毒 系统发生分析 |
| English Key Word: Hemorrhagic fever with renal syndrome Hantaviruses Phylogenetic analysis |
| FundProject:"十五"国家科技支撑计划(2003BA712A08-02);浙江省龙泉市科技局科技项目(2010-017) |
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| Abstract: |
| 目的 调查浙江省龙泉市肾综合征出血热(HFRS)疫源地小动物中汉坦病毒(HV)的自然感染情况及其流行的基因型.方法 用鼠笼在龙泉市查田镇、小梅镇、小梅镇黄南村捕鼠.捕获的小动物进行分类鉴定并解剖取肺脏,用间接免疫荧光法(IFA)检测肺组织中HV抗原,用RT-PCR对HV抗原阳性的样本扩增部分S片段核苷酸序列并测序,构建系统发生树进行基因分型.结果 共捕获小动物319只,其中野外312只,室内7只.黑线姬鼠和东方田鼠为野外优势鼠种.肺组织标本中共检测到9份抗原阳性,带病毒率为4.97%,用部分S片段(620～999nt)的核苷酸序列构建系统发生树,结果表明均为汉滩病毒(HTNV),并与Z251分离株的亲缘关系最近.结论 龙泉市野外主要存在以黑线姬鼠为宿主的HTNV感染,且携带率较高. |
| English Abstract: |
| Objective To investigate the situation of the natural infection of hantaviruses (HV) in small mammals and to provide evidence for the control and prevention of hemorrhagic fever with renal syndrome (HFRS) in Longquan area,Zhejiang province. Methods Small mammals were captured by night trap, and lung tissue samples were collected and stored in liquid nitrogen. HV antigens were detected by indirect immuno-fluorescence assay (IFA). The partial S genome segment sequences were amplified by RT-PCR. DNAStar program was used for editing and comparing the sequences. Phylogeny was analyzed through PAUP*4.0 software. Results 319 small animals were collected in Longquan, and 9 hantavirus antigen-positive samples were identified. The positive rate of hantavirus in Apodemus agrarius was 4.97%. Phylogenetic tree constructed by partial S segment (620-999 nt) showed that the 9 strains carried by A agrarius from Longquan all belonged to HTNV,and had a closer evolutionary relationship with isolate Z251 from Zhejiang province. Conclusion Our results indicated that the main host was A. agrarius and the infection rate of HTNV was high in Longquan area. |
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