| 覃新程,田俊华,王剑波,卢昕,孙强正,金东,周敦金,徐建国,张永振.长角血蜱和微小扇头蜱的形态与分子生物学鉴定[J].Chinese journal of Epidemiology,2011,32(6):608-612 |
| 长角血蜱和微小扇头蜱的形态与分子生物学鉴定 |
| Identification of Haemaphysalis longicomis and Rhipicephalus microplus |
| Received:March 01, 2011 |
| DOI： |
| KeyWord: 长角血蜱 微小扇头蜱 鉴定 |
| English Key Word: Haemaphysalis longicomis Rhipicephalus microplus Identification |
| FundProject: |
| Author Name | Affiliation | E-mail | | QIN Xin-cheng | State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | TIAN Jun-hua | Wuhan Center for Disease Control and Prevention | | | WANG Jian-bo | State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | LU Xin | State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | SUN Qiang-zheng | State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | JIN Dong | State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | ZHOU Dun-jin | Wuhan Center for Disease Control and Prevention | | | XU Jian-guo | State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | ZHANG Yong-zhen | State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | yongzhenzhang@sohu.com |
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| Abstract: |
| 目的 建立鉴定长角血蜱和微小扇头蜱的形态学和分子生物学相结合的方法.方法 采集自湖北和河南省布尼亚新病毒疫区家养动物体表寄生的蜱及草丛、灌木中的蜱;先以形态学鉴定,后用PCR方法扩增得到蜱的12S rDNA,测序后进行同源性和系统进化分析.结果 形态学方法鉴定采集到两种蜱:长角血蜱(Haemaphysalis longicornis)和微小扇头蜱(Rhipicephalus microplus).蜱的12S rDNA经克隆、测序,用PAUP 4.0软件构建系统发生树,湖北、河南省两种蜱的12S rDNA序列分别与长角血蜱和微小扇头蜱聚类,与形态学鉴定结果一致.结论 在传统形态学分类的基础上结合分子生物学鉴定方法能更准确地鉴定蜱的种类. |
| English Abstract: |
| Objective To establish a method combined morphology and molecular marker for identifying Haemaphysalis longicomis and Rhipicephalus microplus. Methods Ticks were collected from domestic animals and wild environment in epidemic area of Hubei and Henan provinces where cases of fever with thrombocytopenia syndrome were prevalent. We classified the ticks by morphology characteristics before 12S rDNA of ticks were amplified by PCR and subsequently sequenced. Phylogenetic tree was constructed by PAUP4.0. Results The ticks belonged to Haemaphysalis longicomis and Rhipicephalus microplus through observation and analysed by the morphological characteristics of the ticks. 12S rDNA was cloned and sequenced while data confirmed the morphological identification of the results. Conclusion The method based on morphology that combined with molecular marker seemed a good method for the identificaton of ticks. |
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