| 王慧珠,焦炳欣,田敬华,李敏,郭杰,刘颖,李兴旺,王玉光.北京、河南、新疆地区HIV/AIDS相关慢性腹泻患者隐孢子虫感染检测分析[J].Chinese journal of Epidemiology,2011,32(9):927-929 |
| 北京、河南、新疆地区HIV/AIDS相关慢性腹泻患者隐孢子虫感染检测分析 |
| Detection of Cryptosporidium infection among HIV/AIDS patients with chronic diarrhea in Beijing, Henan and Xinjiang of China |
| Received:July 07, 2011 |
| DOI： |
| KeyWord: HIV感染者和艾滋病患者 隐孢子虫 慢性腹泻 |
| English Key Word: HIV/AIDS Cryptosporidium Chronic diarrhea |
| FundProject:国家科技重大专项（2008ZX10005-003,2009ZX10005 -014） |
| Author Name | Affiliation | E-mail | | WANG Hui-zhu | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | | | JIAO Bing-xin | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | | | TIAN Jing-hua | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | | | LI Min | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | | | GUO Jie | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | | | LIU Ying | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | | | ULI Xing-wang | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | | | WANG Yu-guang | Beijing Ditan Hospital, Capital Medical University Beijing 100015, China | wygzhyiaids@126.com |
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| Abstract: |
| 目的 了解北京、河南、新疆地区HIV感染者和艾滋病患者(HIV/AIDS)相关慢性腹泻患者中隐孢子虫感染状况。方法收集3个地区HIV/AIDS相关慢性腹泻患者粪便，采用甲醛-乙酸乙酯沉淀法集卵，用改良抗酸染色法染色检测隐孢子虫卵囊。同时检测患者血液CD4+T淋巴细胞计数。结果253例HIV/AIDS相关慢性腹泻患者的粪便标本中有32例为隐孢子虫阳性，感染率为12.6％。北京、河南、新疆地区艾滋病慢性腹泻患者隐孢子虫感染率分别为5.97％(4/67)、16.1％(24/149)和10.8％(4/37)，地区间差异有统计学意义(x2=4.431,P＜0.05)。男性和女性艾滋病慢性腹泻患者隐孢子虫感染率分别为11.2％( 16/1 43)和14.5％(16/110),差异无统计学意义（x2=0.634,P＞0.05）；城市和农村艾滋病慢性腹泻患者隐孢子虫感染率分别为6.5％(7/104)和16.8％(25/149)，差异有统计学意义(x2=5.596,P＜0.05)；夏秋季与其他季节的艾滋病慢性腹泻患者隐孢子虫感染率分别为14.0％(30/214)和5.1％(2/39)，差异无统计学意义(P＞0.05)；艾滋病慢性腹泻患者处于HIV感染无症状期、有症状期、艾滋病期隐孢子虫感染率分别为0％(0/7)、21.3％(19/89)、8.3％( 13/157),差异有统计学意义(x2=9.822，P＜0.01)；不同CD4+T淋巴细胞计数水平（cell/μl）艾滋病慢性腹泻患者隐孢子虫感染率有差异,＜200为20.4％ (20/98),200～499为9.23％(12/130)，≥500为0％( 0/25),差异有统计学意义(x2=10.33,P＜0.01)。艾滋病中晚期慢性腹泻患者，尤其是CD4+T淋巴细胞计数＜200 cell/μl的患者其感染率明显增加。结论3个地区艾滋病慢性腹泻患者隐孢子虫感染率与性别无关；患者来自农村地区、CD4+T淋巴细胞计数水平降低、疾病为中晚期患者易发生隐孢子虫感染。 |
| English Abstract: |
| Objective To investigate the Cryptosporidium infection and its epidemiological characteristics in HIV/AIDS patients with chronic diarrhea.Methods Stool samples collected from HIV/AIDS confirmed patients with chronic diarrhea who lived in Beijing, Henan and Xinjiang.Samples were concentrated by Formalin-Ethyl Acetate Sedimentation technique and stained by modified acid-fast stain (AFS) for the identification of oocysts by microscopy. CD4+T cells count was performed by Flow Cytometry.Results The overall infection rate of Cryptosporidium in AIDS patients was 12.6％(32/253). The infection rates of oocysts in the area of Beijing, Henan and Xinjiangwere 5.97％ ( 4/67 ), 16.1％ (24/149 ) and 1 0.8％ (4/37) respectively. The infection rate of oocysts in the urban areas was 6.5％(7/104) while in the countryside it was 16.8％(25/149) and the difference was significantly different. However, there were no any differences discovered between the infection rates on patient' s gender or on infection occurred in different seasons. The infectious rates of ooeyst in patients on different stages of the disease were also significantly different (P＜0.01).Conclusion AIDS patients infected by Cryptosporidium were not rarely seen in northern China. The rate of infection was not associated with patient' s gender but was associated with patient' s living environments. Patients living in the countryside, with lower lever of CD4+T cells counts and at the middle/late stage of the disease, Cryptosporidium infection appeared to be high. |
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