浙江省部分地区2009--2010年蚊媒中乙型脑炎病毒分子流行病学特征研究

严菊英; 唐学雯; 范飞能; 张意坚; 罗央努; 应永平; 沈静; 张严峻

Abstract

严菊英,唐学雯,范飞能,张意坚,罗央努,应永平,沈静,张严峻.浙江省部分地区2009--2010年蚊媒中乙型脑炎病毒分子流行病学特征研究[J].Chinese journal of Epidemiology,2012,33(1):78-81

浙江省部分地区2009--2010年蚊媒中乙型脑炎病毒分子流行病学特征研究

Study on the molecular characteristics of Japanese encephalitis virus living in vector mosquitoes，in Zhejiang province，2009-2010

Received:July 30, 2011

DOI：

KeyWord: 乙型脑炎病毒基因分型

English Key Word: Japanese encephalitis virus Genotype

FundProject:国家科技重大专项(2009ZXl0004—210)

Author Name	Affiliation	E-mail
YAN Ju-ying	Zhejiang Provincinal Center for Disemse Control and Prevention, Hangzhou 310051, China	Email：jyyanl960@hotmail．com
TANG Xue-wen	Zhejiang Provincinal Center for Disemse Control and Prevention, Hangzhou 310051, China
FAN Fei-neng	Cixi City Center for Disease Control and Prevention
ZHANG Yi-jian	Xianju County Center for Disease Control and Prevention
LUO Yang-nu	Cixi City Center for Disease Control and Prevention
YING Yong-ping	Xianju County Center for Disease Control and Prevention
SHEN Jing	Zhejiang Provincinal Center for Disemse Control and Prevention, Hangzhou 310051, China
ZHANG Yan-jun	Zhejiang Provincinal Center for Disemse Control and Prevention, Hangzhou 310051, China

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Abstract:

目的了解浙江省部分地区蚊媒携带流行性乙型脑炎(乙脑)病毒(JEV)情况及其分子流行病学特征。方法 2009-2010年7-8月从浙江省慈溪市和仙居县乙脑监测点采集蚊13620只，利用荧光定量PCR方法检测蚊中JEV核酸，用BHK-21细胞分离病毒,扩增JEV分离株E基因，然后选择6株进行测序并进行同源性分析。结果慈溪和仙居监测点蚊中JEV带病毒率分别为17.0％(27/159)和3.4％( 1/29)；从2010年143批蚊中分离到22株JEV，分离率为15.4％。6株测序株E基因全长均为1500个核苷酸(nt)，推导编码500个氨基酸(aa)，未发现nt插入和丢失，nt和aa同源性分别为99.2％～ 99.8％和100.0％，与浙江省2007-2008年JEV分离株的nt和aa同源性分别为99.1％～99.3％和99.2％～ 99.8％，与乙脑疫苗株SA14-14-2同源性分别为87.6％～88.0％和97.8％。E基因进化树显示，6株测序株位于基因Ⅰ型分支上。结论浙江省慈溪市和仙居县监测点蚊媒携带JEV，其中慈溪监测点蚊媒中乙脑带病毒率明显高于仙居监测点，JEV分离株均为基因Ⅰ型。

English Abstract:

Objective To investigate the molecular characteristics of Japanese encephalitis virus (JEV) living in vector mosquitoes,from Zhejiang province.Methods A total of 13620 mosquitoes were collected from the monitoring stations located in Cixi city and Xianju county in Zhejiang province,in July and August,2009-2010.Nucleic acid of JEV from the mosquitoes was monitored by using real-time RT-PCR.The virus strains were isolated with BHK-21 cell line,with E genes of the isolated viruses amplified,sequenced and their phylogeny and homology analyzed.Results The positive rates of JEV for those mosquitoes collected in the stations of Cixi and Xianju were 17.0％ (27/159) and 3.4％ ( 1/29 ),respectively.Twenty-two JEV strains were isolated,accounted for 15.4％ among the 143 batches of mosquitoes collected in 2010.All E genes in the 6 sequenced virus isolates contained 1500 nucleotides encoding 500 amino acids,in which no inserts and deletions were identified.The identity rates of nucleotide and amino acid in E gene were 99.2％-99.8％ and 100.0％ among the 6 JEV strains isolated from Zhejiang,99.1％-99.3％ and 99.2％-99.8％ between the Zhejiang strains in 2009-2010 and the Zhejiang strains in 2007-2008,respectively,87.6％-88.0％ and 97.8％ between the 6 Zhejiang strains and the vaccine strain SA 14-14-2 of JEV,respectively.The phylogeny tree of E gene indicated that the JEV isolates in Zhejiang during 2009-2010 was located in the branch of the genotype Ⅰ.Conclusion Mosquitoes collected from Cixi and Xianju areas carried JEV,with the rate of JEV in Cixi higher than in Xianju.All the Zhejiang isolates in 2009-2010 were proven to be the genotype Ⅰ of JEV.

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