| 唐琨,左双燕,李颖,郑元春,霍秋波,于季红,张圆,倪雪冰,姚囡囡,谭红专.黑龙江省旅游区蜱伯氏疏螺旋体和斑点热群立克次体复合感染的动态调查[J].Chinese journal of Epidemiology,2012,33(5):513-516 |
| 黑龙江省旅游区蜱伯氏疏螺旋体和斑点热群立克次体复合感染的动态调查 |
| Dynamic investigation on the co-infection status of two pathogens in ticks from tourist point in Heilongjiang province |
| Received:November 28, 2011 |
| DOI： |
| KeyWord: 伯氏疏螺旋体 斑点热群立克次体 复合感染 序列分析 |
| English Key Word: Borrelia burgdorferi sensu lato Spotted fever group Rickettsiae Co-infection Sequence analysis |
| FundProject:国家重点基础研究发展计划(973计划)(2010CB530201) |
| Author Name | Affiliation | E-mail | | Tang Kun | Department of Epidemriology and Health Statistics, School of PublicHealth, Gentraf South University, Changsha 410078, China | | | Zuo Shuangyan | Department of Epidemriology and Health Statistics, School of PublicHealth, Gentraf South University, Changsha 410078, China | | | Li Ying | School of Architecture and Urban Environment, Soochow University | | | Zheng Yuanchun | Forestry Center Hospital of Mudanjiartg | | | Huo Qiubo | Forestry Center Hospital of Mudanjiartg | | | Yu Jihong | Department of Infection Management and Disease Control Chinese PLA GeneralHospital | | | Zhang Yuan | Department of Epidemriology and Health Statistics, School of PublicHealth, Gentraf South University, Changsha 410078, China | | | Ni Xuebing | Department of Epidemriology and Health Statistics, School of PublicHealth, Gentraf South University, Changsha 410078, China | | | Yao Nannan | Forestry Center Hospital of Mudanjiartg | | | Tan Hongzhuan | Department of Epidemriology and Health Statistics, School of PublicHealth, Gentraf South University, Changsha 410078, China | tanhz99@qq．com |
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| Abstract: |
| 目的了解黑龙江省旅游庆蜱伯氏疏螺旋体和斑点热群立克次体复合感染的动态变化。方法运用PCR方法检测2010年捕获蜱中两种病原感染状况并测序进行序列分析。结果在威虎山和镜泊湖景区采集点捕获蜱标本共849只。两地优势蜱种分别为全沟硬蜱和嗜群血蜱，其中伯氏疏螺旋体和斑点热群立克次体感染率分别为26．15％、10．05％和0．00％、13．33％。提示全沟硬蜱复合感染。两地优势蜱种活动高峰分别出现在7月和6月上旬，蜱携带病原率因时问差异而不同。序列分析显示旅游区伯氏疏螺旋体有3种基因型，除丑garinii型、&afzelii型外，首次在东北地区发现丑va／a／s／ana—like group型。对斑点热群立克次体序列分析表明，所得两序列分别与Rickettsia sp，IIL一93 和Rickettsiasp．H820，其同源性为100％。结论两旅游Ⅸ蜱中存在伯氏疏螺旋体和斑点热群立克次体复合感染。且携带的基因类型存在多样性；动态调查显示蜱种、蜱密度的分布以及蜱携带病原状况因生境、时间和人群的不同而有差异。 |
| English Abstract: |
| 0bjective To monitor the co—infecfions tatus of Borrelia burgdorferi sensu lato(B.b.S.l)and spotted fever group Rickettsia(SFGR)in tourist areas of Heilongjiang province.Methods Polymerase chain reaction(PCR)was used to detect the 5S-23S rRNA intergenic spacer of B.b.S.1 and ompA of SFGR in ticks.dynamically collected from tourist areas of Heilongjiang provincein 2010.Amplification products from positive ticks were sequenced.and phylogenetic analysis was conducted by Mega 5.0 software package.Results 849 ticks were collected from two tourist points,with the dominant ticks in Tiger Motmtain and Jingpo Lake were lxodes persulcatus and Haemaphysalis concinna.Regarding the bodes persulcatus from Tiger Mountain,the infection rates of B.b.s.1 and SFGR were 26.15％and 10.05％.The infection rate of SFGR was 13-33％in Haemaphysalis concinna and the B.b.s.1 was undiscovered in the same ticks from Jingpo Lake.However,the co-infection could only be detected in lxodes persulcatus of both tourist areas.Surveillance datashowed that the major tieks were morel ikely to be appeared in July at Tiger Mountain and in June at Jingpo Lake.Data from the sequence analysis on B.b.S.l showed that the B.b.S.1 in tourist areas could be classified into three different genotypes other than B.gariniiand B.afzelii.We first detected B.valaisiana—like group genotypei in northeast of China.Results from the sequence analysis of SFGR positive products showed that the two DNA sequences of newlyd etected agents were completely the sanlc as Rickettsiasp.HL一93 which was detected in Hulin and Rickeus／asp.H820 found in northeast China Conclusion The CO-infection of b.s.l and SFGR was detected in ticks from the tourist areas of Heilongliang province and data from the sequencing of specific fragment showed that varinus kinds of genotypes existed in this area.However,the rates of co-infectionitis—different according to environment time and population that contributed to the kinds of and the index ofticks existed in the surveys points,also the infection rate of the ticks was studied. |
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