Abstract
张秀春,张立霞,李伟红,王誓闻,孙玉兰,王圆圆,关增智,刘秀军,杨育松,张曙光,禹惠兰,张丽娟.北京市农村人群及家畜嗜吞噬无形体和查菲埃立克体感染状况调查[J].Chinese journal of Epidemiology,2012,33(5):517-520
北京市农村人群及家畜嗜吞噬无形体和查菲埃立克体感染状况调查
Investigation on Ehrlichia ehaffeensis and Anaplasma phagocytophilum infection among farmers and domestic animals in rural areas of Beijing China
Received:December 30, 2011  
DOI：
KeyWord: 菲埃立克体  嗜吞噬元形体  血清流行病学  间接免疫荧光方法
English Key Word: Ehrlichia chaffevnsis  Anaplasma phagocytophilum  Seroprevalence  Micro-indirect immunofluorescent assay
FundProject:同家重点基础研究发展计划(973计划)(2010CB530200．2010CB530206)；传染病防治科技重大专项一细菌性传染病病原谱流行规律与变异研究(2009ZXl0004-203)及病原体实验事网络化监测技术研究(2008ZXl0004—008)
Author NameAffiliationE-mail
Zhang Xiuchun Beijing Center for Disease Control and Prevention Beijing 100013 China  
Zhang Lixia Natiohal Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention  
Li Weihong Beijing Center for Disease Control and Prevention Beijing 100013 China  
Wang Shiwen Natiohal Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention  
Sun Yulan Beijing Center for Disease Control and Prevention Beijing 100013 China  
Wang Yuanyuan Natiohal Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention  
Guan Zengzhi Beijing Center for Disease Control and Prevention Beijing 100013 China  
Liu Xiujun Tongzhou District Center for Disease Control and Prevention Beijing  
Yang Yusong Miyun County Center for Disease Control and Prevention Beijing  
Zhang Shuguang Yangqing Countr Center for Disease Control and Prevention Beijing  
Yu Huigan Natiohal Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention  
Zhang Lijuan Natiohal Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention zhanglijuan@icdc．cn 
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Abstract:
      目的调查北京市农村人群及家畜新发查菲埃立克体和嗜吞噬无形体感染状况。方法按地理位置选择北京市西北(延庆)、东北(密云)及东南(通州)地区3个植被率较高的旅游县(区)。按在册户籍随机以家庭为单位，调查并采集562人及90只山羊、71头牛和2只家犬血液标本，按WHO推荐间接免疫荧光方法(mlFA)检测血清lgG抗体。动物血球提取DNA扩增无形体16SrRNA基因并测序分析。结果人群查菲埃立克体和嗜吞噬无形体抗体阳性率分别为16．5％和14．O％；山羊和牛嗜吞噬无形体抗体阳性率分别为2．3％和O；2只家犬未检测到相应抗体。山羊、牛无形16SrRNA扩增阳性率分别为48．9％和23．9％16SrRNA序列(228bp)分析显示，3类优势流行株无明显地理分布特征。结论北京市农村人群存在查菲埃立克体和嗜吞噬无形体感染．调查地区家畜存在无形体感染。
English Abstract:
      0bjectiveTo investigate the status of Ehrlichia(E)chaffeensis and Anaplasma(A.)phagocytophilum infection among farming populations and domestic animals in the mral area of BeOing,China.Methods Blood samples from 562 farmers and 163 blood samples including 90 goats,71 ox and 2 dogs were collected.Specificityof lgG antibodies against E.chaffeensis and A.phagocytophilum were tested by micro-indirect irm-nunofluorescent assay(mIFA).16SrRNA genes of A.phagocytophilum were amplificd from the domestic animal blood samples and products were sequenced and analyzed by nested PCR.Results The positive rates of E chaffeensis and A.phagocytophilum antibody were 16.5％and 14.O％respectively for farmers.The total positive rates of A.phagocytophilum were 2.3％and 0 for both goats and oxen respectively.No antibody was found for the 2 tested dogs.The PCR positive rates were 48.9％and 23.9％for goats and oxen respectively.Three dominant varieties of A.phagocytophilum were demonstrated in goats and oxen.ConclusionThe prevalence rates of E.chaffeensis and A.phagocytophilum were identified in the rural areas of Beijing.
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