| 王峰,胡思玉,桂静,崔运勇,刘小立,李庆阁.PCR熔解曲线法筛查结核分枝杆菌链霉素和乙胺丁醇耐药性[J].Chinese journal of Epidemiology,2012,33(5):525-528 |
| PCR熔解曲线法筛查结核分枝杆菌链霉素和乙胺丁醇耐药性 |
| A rapid screening program on the resistance to streptomycin and ethambutol in Mycobacterium tuberculosis isolates by PC Rmelting curve analysis |
| Received:October 09, 2011 |
| DOI： |
| KeyWord: 结核分枝杆菌 耐药性 链霉素 乙胺丁醇 |
| English Key Word: ycobacterium tuberculosis Drug resistance Streptomycin Ethambutol |
| FundProject:国家“十一五”科技重大专项(2008ZXl0003一004) |
| Author Name | Affiliation | E-mail | | Wang Feng | Departmem of Pathogenic Laboratory, Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China | | | Hu Siyu | Engineering Research Center of Molecular Diagnostics, Ministry of Education, School of Science, Xiamen University | | | Gu Jing | Departmem of Pathogenic Laboratory, Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China | | | Cui Yunyong | Departmem of Pathogenic Laboratory, Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China | | | Liu Xiaoli | Departmem of Pathogenic Laboratory, Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China | liuxl36@126．Com | | Li Qingge | Engineering Research Center of Molecular Diagnostics, Ministry of Education, School of Science, Xiamen University | qgli@xmu.edu．cn |
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| Abstract: |
| 目的利用PCR熔解曲线法快速筛查结核分枝杆菌(MTl3)对链霉素、乙胺丁醇耐药性，并与药敏试验结果比较，评价其应用价值。方法收集深圳市2007--2009年331株MTB临床分离株，应用PCR熔解曲线法检测embB基因306、378—380、406和497、rpsL基因43、88及m基因513—517、905—908位点耐药突变，并与药敏试验结果比较。结果以药敏试验结果为标准，PCR熔解曲线法检测MTB链霉素耐药突变的敏感性为78．6％、特异性为90．1％、准确性为86．7％；检测MTB乙胺丁醇耐药突变的敏感性为83．o％、特异性为93．3％、准确性为91．8％。PCR熔解曲线法与药敏试验检测结果具有很好的一致性。结论PCR熔解曲线法能够快速、特异地检测MTB对链霉素、乙胺丁醇耐药突变，可用于临床筛查。 |
| English Abstract: |
| Objective To evaluate the effects of PCR melting curve analysis assay on arapid screening program regarding the resistance of Mycobacterium tuberculosis(MTB)clinical isolates to streptomycin and ethambutol.MethodsA total of 331 clinical isolates of MTB had been collected since 2007—2009 in Shenzhan.Mutations at codon 306,378—380,406and 497 of embB gene,codon 43,88 ofrpsl gene,and 513-517,905-908 region of rrs gene were detected by PCR melting curve analysis.Result were compared with that of conventional drug susceptibility test.ResultsCompared to drug susceptibility test,sensitivity, specificity and accuracy for streptomycin resistance were 78.6％,90.1％and86.7％,respectively while83.O％,93.3％and91.8％,respectively for etharnbutol resistance detected by PCR melting curve analysis.PCR melting curve method was in good agreement with drug susceptibility test.Conclusion PCR melting curve analysis on genetic regions associated with resistance to streptomycin and ethambutol seemed to be arapid,specific and closed-tube method so it could be used for detection of treptomycin and ethambutol resistance in MTB. |
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