| 左双燕,唐琨,李颖,于季红,张圆,倪雪冰,郑元春,霍秋波,宋玉东,曾小敏.黑龙江林区野鼠伯氏疏螺旋体核酸检测与序列分析[J].Chinese journal of Epidemiology,2012,33(6):643-644 |
| 黑龙江林区野鼠伯氏疏螺旋体核酸检测与序列分析 |
| DNA detection and sequence analysis of Borrelia burgdorferi sensu lato in rodents from Helongjiang forest region |
| Received:November 28, 2011 |
| DOI： |
| KeyWord: 伯氏疏螺旋体 序列分析 |
| English Key Word: Borrelia burgdorferi sensu lato Sequence analysis |
| FundProject:国家重点基础研究发展计划(973计划)(2010CB530201);国家杰出青年基金(30725032) |
| Author Name | Affiliation | E-mail | | ZUO Shuang-yan | Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China | | | TANG Kun | Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China | | | LI Ying | School of Architecture and Urban Environment, Soochow University | | | YU Ji-hong | Department of Infection Management and Disease Control, General Hospital of the People's Liberation Army | | | ZHANG Yuan | Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China | | | NI Xue-bing | Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China | | | ZHENG Yuan-chun | Forestry Center Hospital, Mudanjiang City, Helongjiang Province | | | HUO Qiu-bo | Forestry Center Hospital, Mudanjiang City, Helongjiang Province | | | SONG Yu-dong | Forestry Center Hospital, Mudanjiang City, Helongjiang Province | | | ZENG Xiao-min | Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China | zxiaomin@xysm.net |
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| Abstract: |
| 伯氏疏螺旋体共有13个基因种, 国内分离到的菌株包括5个基因种[1-3], 本研究对黑龙江地区啮齿动物中伯氏疏螺旋体进行分子流行病学调查和分析. 1.材料与方法: (1)样本采集、处理及DNA提取:2009-2011年4-7月从黑龙江林区采用夹夜法采集野鼠, 每样点100×2夹次, 按5m距离布放鼠夹, 晚放晨收.现场鉴定鼠种后, 将捕获的鼠放入鼠袋内, 乙醚麻醉后, 消毒, 无菌取脾脏低温保存.采用天根生化科技(北京)有限公司血液/细胞/组织基因组DNA提取试剂盒提取脾脏DNA, -20℃贮存备用. |
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