焦永卓,牟李红,王应雄,晏维,蒋苗.成釉细胞蛋白基因多态性与氟斑牙关系的研究[J].Chinese journal of Epidemiology,2013,34(1):28-32 |
成釉细胞蛋白基因多态性与氟斑牙关系的研究 |
Association between ameloblastin gene polymorphisms and the susceptibility to dental Fluorosis |
Received:July 04, 2012 |
DOI:10.3760/cma.j.issn.0254-6450.2013.01.007 |
KeyWord: 氟斑牙 成釉细胞蛋白 燃煤污染 基因多态性 |
English Key Word: Dental fluorosis Ameloblastin Coal-fired polluting Gene polymorphism |
FundProject:重庆市地质矿产勘探开发局资助([2010]科函02号) |
Author Name | Affiliation | E-mail | Jiao Yong-zhou | Department of Epidemiology, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China | | Mu Li-hong | Department of Epidemiology, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China | mulihong@yahoo.com | Wang Ying-xiong | Faculty of Basic Medical Sciences, Chongqing Medical University | | Yan Wei | Institution of Endemic Disease Prevention, Chongqing Center for Disease Control and Prevention | | Jiang Miao | Department of Epidemiology, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China | |
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Abstract: |
目的观察成釉细胞蛋白(AMBN)基因多态性在重庆市燃煤型氟中毒人群中的分布,探讨AMBN基因多态性与氟斑牙的关系。方法采用病例对照研究,在重庆市巫山、奉节县2个燃煤型氟中毒病区抽取8~12岁氟斑牙患病儿童100例、成人30例作为病例组;分别抽取非氟斑牙患病8~12岁儿童100例、成人30例作为内对照组,另在渝北区(非病区)抽取50名儿童、30名成人作为外对照组。采集所有研究对象外周静脉血,提取DNA,采用聚合酶链反应一限制性片段长度多态性(PCR-RFLP)方法测定3组人群AMBN基因7号外显子(538_ 540de1GGA),10号外显子(657A > G)和13号外显子(986C>T)位点基因型,观察并分析比较组间各基因型分布差异。结果病例及内、外对照组GGA/GGA基因频率分别为61.2%(74/121),78.5%(102/130)和74.3%(52/70 ), GGA/-基因频率分别为24.0% ( 29/121),15.4% ( 20/130)和22.9% (16/70 ),-/--(GGA完全缺失)基因频率分别为14.8%(18/121),6.1%(8/130)和2.8% ( 2/70 ),各组间的差异有统计学意义( x2=14.353, P=0.006 );病例及内、外对照组AA基因频率分别为86.8%(105/121),93.1%(121/130)和91.4% ( 64/70 ), AG基因频率分别为13.2%(16/121),6.9%(9/130)和8.6% ( 6/70 ),各组间差异无统计学意义(x2=2.972,P>0.05);病例及内、外对照组CC基因频率分别为81.0% ( 98/121),90.0% (117/130)和87.1%(61/70);CT基因频率分别为19.0% ( 23/121),10.0% (13/130)和12.9% ( 9/70),各组间差异无统计学意义(x2=4.319,P>0.05)。与两对照组相比,病例组的GGA/GGA基因型频率降低(x2值分别为8.957,3.405,P值均<0.05 ), GGA完全缺失基因型频率增高(才值分别为5.134,6.833,P值均<0.05。单因素分析显示,携带一基因型的个体发生氟中毒的风险增高(病例组与内、外对照组比较,OR值分别为2.7, 5.9, P值均<0.05 )。与内对照组比较,病例组CT基因型频率增高(x2=4.139,P<0.05)。病区携带CT基因型的个体发生氟中毒的风险增高(OR=2.1,P< 0.05 )。结论AMBN基因7号外显子538_540de1GGA和13号外显子986C>T位点多态性可能是影响氟斑牙发病的易感性因素之一。 |
English Abstract: |
Objective To study the distribution of ameloblastin(AMBN) gene polymorphism in coal-fire caused fluorosis (CFCF) in Chongqing municipality and the relationship between AMBN gene polymorphism and the susceptibility to dental fluorosis. Methods Under a case-control study,100 children aged 8-12 and 30 adults with dental fluorosis were enrolled in Wushan and Fengjie counties of Chongqing from December 2010 to February 2011. Another 100 children aged 8-12 and 30 adults with non-dental fluorosis were chosen as internal control groups together with 50 childrenand 30 adults without dental fluorosis were selected as external control groups in the non-epidemic area of Yubei district. DNA was extracted from peripheral blood sample of these people. Genotype of AMBN gene 7 extron 538_540de1GGA, 10 extron 657A>G and 13 extron 986C>T loci were detected using the polymerise chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results The rates of 7 extron 538_540delGGA loci among case, internal and external control groups were as follows: GGA/GGA-/-61.2% ( 74/121),78.5%(102/130 ),74.3% ( 52/70 );GGA/-:24.0% (29/121),15.4% (20/130),22.9% (16/70 );-/-:14.8% (18/121),6.1% (8/130), 2.8% ( 2/70 ),the difference was statistically significant(x2=14.353,P<0.05). The AA appeared to be 86.8% 105/121),93.1%(121/130),91.4%(64/70) andAGwere 13.2%(16/121),6.9%(9/130),8.6% (6/70),with difference not statistically significant (x2=2.972, P>0.05). CC appeared as 81.0%(98/121),90.0% (117/130 ),87.1%(61/70) while CT as 19.0%(23/121),10.0% (13/130 ),12.9% ( 9/70 ),with difference not statistically significant (x2=4.319, P>0.05). In comparing with the two control groups, the frequency of GGA/GGA was decreasing (x2 values were 8.957, 3.405,respectively, P<0.05 ) while the frequency of-/-- was increasing(x2 values were 5.134, 6.833, respectively, P<0.05). Results from the univariate analysis showed that the individuals who were carrying-/-genotype had an increased risk of suffering from fluorosis (OR values were 2.7, 5.9, respectively, P<0.05). When compared with the internal control group, the CT genotype of case group showed an increase (x2=4.139, P<0.05) while individuals that carrying CT genotype had an increased risk of suffering from fluorosis ( OR=2.1,P< 0.05 ),in epidemic-area. Conclusion Our Results showed that the 7 extron 538-40de1GGA and the 13 extron 986C>T loci polymorphism in AMBN gene might serve as the susceptibility factors causing the coal-fired fluorosis. |
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