| 张佳峰,潘晓红,丁晓贝,陈琳,郭志宏,徐云,黄晶晶.浙江省2009年随访HIV/AIDS的分子流行病学研究[J].Chinese journal of Epidemiology,2013,34(1):44-48 |
| 浙江省2009年随访HIV/AIDS的分子流行病学研究 |
| Molecular epidemiological study on HIV/AIDS under the follow-up program in Zhejiang province in 2009 |
| Received:September 19, 2012 |
| DOI：10.3760/cma.j.issn.0254-6450.2013.01.012 |
| KeyWord: 艾滋病病毒 亚型 分子流行病学 |
| English Key Word: Human immunodeficiency virus Subtype Molecular epidemiology |
| FundProject:浙江省科技计划(2009033145) |
| Author Name | Affiliation | E-mail | | Zhang Jia-feng | Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China | | | Pan Xiao-hong | Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China | xhpan310009@yahoo.com.cn | | Ding Xiao-bei | Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China | | | Chen Lin | Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China | | | Guo Zhi-hong | Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China | | | Xu Yun | Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China | | | Huang Jing-jing | Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China | |
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| Abstract: |
| 目的分析2009年浙江省随访艾滋病病毒感染者戊滋病患者(HIV/AIDS)分子流行病学特征。方法以浙江省各地市为单位随机抽样确定303例研究对象，收集个案信息、采集样本并提取基因组DNA，用巢式PCR扩增gdg基因片段，测序并进行生物信息学分析。结果序列成功获得率为74.3% (225/303 )。存在7种亚型，分别为CRFO1_AE (58.7% ), CRF07_BC(13.8%),CRF08_BC(9.8%),B'(15.1%>,C(l.8%>,G(o.4%)和未定义Bc重组(新型独特重组型，0.4% ) o HIV BLAST分析显示，毒株同源性最高的报告地涉及国内辽宁、广西、云南、河南等10个省(区)和泰国、越南、印度、南非和利比亚5个国家。CRFO1_AE系统进化树分为4簇，同性传播感染者序列聚集于簇1，但与异性传播感染者混合并存。结论浙江省HIV毒株的多样性和复杂性进一步增加。以流行重组型占显著优势，存在新型独特重组型和新型别，同性传播与异性传播感染者呈交叉渗透趋势。 |
| English Abstract: |
| Objective To analyze the molecular epidemiological characteristics on HIV infectors/AIDS patients (HN/AIDS) under a follow-up program in Zhejiang province in 2009. Methods 303 cases were randomly sampled. Information on the cases was collected and followed by genomic DNA extraction. Gag gene fragments were amplified by nested PCR, followed by sequencing and bio-informatic analysis. Results The rate of success for sequence acquisition was 74.3% (225/303). Distributions of HIV subtypes were as follows: CRFO1_AE ( 58.7%)，CRF07_BC(13.8%)，CRF08_BC ( 9.8%)，B'(15.1% )，C (1.8%)，G ( 0.4%)and unassigned BC(unique recombinant form 0.4%).Results from the HN BLAST analysis showed that the sources of strains with the highest homology involved in 10 provinces/municipalities (Liaoning, Guangxi, Yunnan, Henan, etc. ) and five other countries (Thailand, Vietnam, India, South Africa and Libya). The CRFO1_AE phylogenetic tree was divided into four clusters. The sequences of HN/AIDS with homosexual transmission showed a gathering in cluster 1，and mixing with those infected through heterosexual contact. Conclusion Circulating recombinant forms of HIV seemed to play a dominant role in Zhejiang province. Unique recombinant form and new subtype of HIV were found. People living with HIV under homosexual transmission and heterosexual transmission had a trend of interwoven with each other. Increase of both the diversity and complexity of HIV strains were also noticed in Zhejiang province. |
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