广西壮族自治区2008 - 2009年HIV-1流行毒株基因型及其分布

刘伟; 梁淑家; 杨进业; 李剑军; 王斌; 陈立力; 李林; 刘永健; 李敬云

Abstract

刘伟,梁淑家,杨进业,李剑军,王斌,陈立力,李林,刘永健,李敬云.广西壮族自治区2008 - 2009年HIV-1流行毒株基因型及其分布[J].Chinese journal of Epidemiology,2013,34(1):53-56

广西壮族自治区2008 - 2009年HIV-1流行毒株基因型及其分布

Distribution of HIV-1 subtypes in Guangxi Zhuang Autonomous Region, 2008-2009

Received:July 17, 2012

DOI：10.3760/cma.j.issn.0254-6450.2013.01.014

KeyWord: 艾滋病病毒1型 HIV基因亚型分子流行病学

English Key Word: Human immunodeficiency virus type 1 Genetic subtype Molecular epidemiological distribution

FundProject:国家科技重大专项(2008ZX10001-004)

Author Name	Affiliation	E-mail
Liu Wei	Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China
Liang Shu-jia	Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China
Yang Jin-ye	Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China
Li Jian-jun	Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China
Wang Bin	Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China
Chen Li-li	Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology, Academy of Military Medical Science Urumqi GeneralHospital of Lanzhou Military Area Command
Li Lin	Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology, Academy of Military Medical Science
Liu Yong-jian	Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology, Academy of Military Medical Science
Li Jing-yun	Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology, Academy of Military Medical Science	lijy@nic.bmi.ac.cn

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Abstract:

目的了解广西地区目前HIV-1流行毒株的基因型及其分布。方法收集广西地区13个市294名2008-2009年新诊断的HIV感染者血浆标本和背景信息，感染途径主要为异性性传播(86.1%)。采用RT PCR法分别扩增HIV-1 gag,pol全长基因(分别为1584 by和3147 by)及enu:基因的C2V3片段(558 bp)，序列编辑后用Genotyping及Mega 5.031具确定病毒的基因型，用Simplot和Recombinant HIV-1 Drawing Tool软件进行病毒基因的重组分析。结果获得全长gag基因序列270条、全长pol基因序列246条、。enu>基因C2V3区序列223条，确定272例标本的基因型。CRFO1_AE占的比例最高(77.6% )，其次为CRF08_BC(10.7%)和CRF07_BC ( 7.4% )，有4例B(B')亚型,1例G亚型，还有7例未知重组型。性别、民族间病毒基因型分布的差异无统计学意义。7株新型重组毒株有6株以CRF01_AE为母株，嵌人B和/(或)C的片段，还有1株为CRF07_BC和CRF08_BC的二代重组。结论广西地区目前流行的HIV-1毒株以CRFO1_AE为主，并出现以CRFO1_AE为母株嵌人其他毒株基因片段的新型重组毒株，呈现基因组结构复杂化趋势。

English Abstract:

Objective To understand the distribution of HIV-1 subtypes in Guangxi Zhuang Autonomous Region. Methods 294 participants who were infected by HIV-1 in 2008-2009 and residing in 13 cities in Guangxi were enrolled into this study. pidemiological information showed that heterosexual transmission was the main transmitting route. 1584 by full length gag gene, 3147 by full length pol gene and 558 bp env ( C2V3 ) fragments were amplified by using RT PCR and then directly sequenced. NCBI genotyping tool and Mega 5.03 software were used to determine the HIV subtypes.Simplot and Recombinant HIV-1 Drawing Tool were used for the analysis of recombination. Results A total of 739 sequences, including 270 gag, 246 pol and 223 env (C2V3 )，were successfully obtained from 294 plasma specimens. Genotypes of HIV from 272 participants were determined. CRF01_AE was found the dominant ( 77.6%)，followed by CRF08_BC (10.7% )and CRF07_BC(7.4%). 7 unique recombinant forms, 4 subtype B(B')and 1 subtype G were also identified. No significant difference on the distribution of subtypes among different regions and ethnics was found. Among the 7 unknown recombinant forms, 6 strains were mosaic B and /or C fragments with CRFO1ee AE genome as the backbone, while one strain originated from CRF07_BC and CRF08_BC. Conclusion Currently,CRF01_AE was found the major subtype of HIV epidemic in Guangxi. New recombinant Forms with CRF01_AE as backbones had been emerging, which called for serious attention.

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