| 苏文莉,陈琛,王中强,李婧,何湘,孙走南,杨益,刘京梅,邱少富,王勇,宋宏彬.北京地区福氏志贺菌Xv新亚型毒力基因与致病力研究[J].Chinese journal of Epidemiology,2013,34(1):57-60 |
| 北京地区福氏志贺菌Xv新亚型毒力基因与致病力研究 |
| Virulence and pathogenicity of Shigella flexneri Xv isolated in Beijing |
| Received:July 09, 2012 |
| DOI：10.3760/cma.j.issn.0254-6450.2013.01.015 |
| KeyWord: 福氏Xv志贺菌 毒力基因 侵袭力 |
| English Key Word: Shigella flexneri Xv Virulence gene Invasiveness |
| FundProject:国家自然科学基金(81001267/H2609);北京市科技新星项目(2010B067) |
| Author Name | Affiliation | E-mail | | Su Wen-li | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | | | Chen Chen | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China
Tai’an Center for Disease Control and Prevention,Shandong | | | Wang Zhong-qiang | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | | | Li Jing | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | | | He Xiang | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | | | Sun Zuo-nan | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | | | Yang Yi | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | | | Liu Jing-mei | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | | | Qiu Shao-fu | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | qiushf0613@hotmail.com | | Wang Yong | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | ywang7508@yahoo.com.cn | | Song Hong-bin | Institute of Disease Control and Prevention Academy of Military Medical Sciences, Beijing 100071，China | hongbinsong@163.net |
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| Abstract: |
| 目的了解北京地区福氏志贺菌Xv新亚型生化特征、毒力基因与致病力。方法对分离自北京地区的61株福氏Xv新亚型志贺菌采用API生化板条鉴定，血清分型采用玻片凝集法，应用PCR技术检测其ipaH,sen,virF和ial毒力基因，采用脉冲场凝胶电泳(PFGE )了解分型特征，以鼠肺切片检测其致病力。结果61株福氏Xv志贺菌只发酵葡萄糖、甘露醇、密二糖和阿拉伯糖;血清凝集抗原结构式为(N : 7, 8); ipaH, sen, virF和ial，毒力基因携带率分别为100%,81.97%, 75.41%和80.30%;全部菌株PFGE分型可分为25种带型，鼠肺切片显示呈炎性改变。结论福氏Xv志贺菌毒力基因携带率高，表现出遗传多态性，具有侵袭力。 |
| English Abstract: |
| Objective To understand the biochemical characteristics, virulence genes and pathogenicity of Shigella flexneri Xv isolated in Beijing. Methods 61 strains of S. flexneri Xv isolated from diarrhea patients in Beijing were systematically determined through biochemical reactions and serological tests. Application of PCR technique in detection of virulence genes on ipaH, sen, virF, ial and pulsed-field gel electrophoresis (PFGE) was used to identify the related characteristics and on rat lung slices to determine its pathogenicity. Results All of the S. flexneri Xv could ferment glucose, mannitol, melibiose and arabinose. Using serum agglutination, we found that the antigen structure was(N:7, 8). IpaH, sen, virF and ial that carried rates of virulence genes appeared to be 100%，81.97%，75.41 % and 80.30%，respectively. Among 61 strains of S. flexneri Xv,the PFGE typing of Shigella bacteria could be divided into 25 belt types while the Results from rat lung
slices showed inflammatory change of Xv. Conclusion S. flexneri Xv was found that it carried high rate of Shigella virulence genes, exhibiting genetic polymorphism and highly invasive. |
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